![Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Western-Blot-H00010736-M01-img0008.jpg "Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, KD |
| Clone | 3D7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | SIX2 (AAH24033, 1 a.a. ~ 291 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS |
| Specificity | SIX2 - sine oculis homeobox homolog 2 (Drosophila) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIX2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and RNAi Validation. Use in FLOW reported in scientific literature (PMID: 26295710). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23996934). |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ and hFK serum-free cultures. Mouse embryonic kidney organ culture stained for Ncam1, Six2 and E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with and processed in ImageJ/Fiji software. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Immunocytochemistry-Immunofluorescence-H00010736-M01-img0010.jpg "Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ and hFK serum-free cultures. Mouse embryonic kidney organ culture stained for Ncam1, Six2 and E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with and processed in ImageJ/Fiji software. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.")
![Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi ( Cat # H00010736-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIX2 monoclonal antibody (M01), clone 3D7 (Cat # H00010736-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Western-Blot-H00010736-M01-img0006.jpg "Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi ( Cat # H00010736-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIX2 monoclonal antibody (M01), clone 3D7 (Cat # H00010736-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulation. Double labelling of sorted NCAM1+ cells for NCAM1 and SIX2: NCAM1 with DAPI (upper panel; red and blue channels), NCAM1 with SIX2 (lower panel; red and green channels), indicating both NCAM1+ SIX2+ (arrows) and NCAM1+ SIX2- (arrowheads) cells. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Immunocytochemistry-Immunofluorescence-H00010736-M01-img0011.jpg "Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulation. Double labelling of sorted NCAM1+ cells for NCAM1 and SIX2: NCAM1 with DAPI (upper panel; red and blue channels), NCAM1 with SIX2 (lower panel; red and green channels), indicating both NCAM1+ SIX2+ (arrows) and NCAM1+ SIX2- (arrowheads) cells. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.")
![ELISA: SIX2 Antibody (3D7) [H00010736-M01] - Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-ELISA-H00010736-M01-img0009.jpg "ELISA: SIX2 Antibody (3D7) [H00010736-M01] - Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.")
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415485112.jpg "Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415482241.jpg "Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ & hFK serum-free culturesMouse embryonic kidney organ culture stained for Ncam1, Six2 & E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with & processed in ImageJ/Fiji software.Morphology of hFK cells cultured in SFM or SCM after 3 days (passage 0 Day 3—left panels) & towards confluence (14 days in SFM or 7 days in SCM—right panels). Distinct borders appear in SFM cultures (arrows) whereas cells with different morphology (arrows) are observed in SCM culture. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.qRT-PCR analysis of gene expression in hFK cells cultured in SFM (three independent replicates). hPRT1 was used as endogenous control & SCM cells were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software.Representative FACS analysis of NCAM1 expression in hFK cells cultured in SFM at passage1. Data is presented in a histogram graph showing NCAM1 staining in blue & the isotype controls staining (negative control) in red.Immunofluorescence staining of NCAM1 (red) in total hFK cells cultured in SFM. Nuclei stained with Dapi (blue). Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415482281.jpg "Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ & hFK serum-free culturesMouse embryonic kidney organ culture stained for Ncam1, Six2 & E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with & processed in ImageJ/Fiji software.Morphology of hFK cells cultured in SFM or SCM after 3 days (passage 0 Day 3—left panels) & towards confluence (14 days in SFM or 7 days in SCM—right panels). Distinct borders appear in SFM cultures (arrows) whereas cells with different morphology (arrows) are observed in SCM culture. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.qRT-PCR analysis of gene expression in hFK cells cultured in SFM (three independent replicates). hPRT1 was used as endogenous control & SCM cells were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software.Representative FACS analysis of NCAM1 expression in hFK cells cultured in SFM at passage1. Data is presented in a histogram graph showing NCAM1 staining in blue & the isotype controls staining (negative control) in red.Immunofluorescence staining of NCAM1 (red) in total hFK cells cultured in SFM. Nuclei stained with Dapi (blue). Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")