OPA1 Antibody (1284B) - BSA Free

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Under conditions of stress, dynamin-like 120 kD protein, form S1 (which is the inactive form produced via cleavage at S1 position by OMA1) triggers the loss of mitochondrial membrane potential that results in negative regulation of the process of mitochondrial fusion. OPA1 exists as oligomeric complex consisting of membrane-bound and soluble forms of OPA1, and the latter can interacts with CHCHD3, IMMT and PARL. PARL-dependent proteolytic processing releases an antiapoptotic soluble form which is not required for mitochondrial fusion. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IF/IHC, WB.

Showing Publications 1 - 7 of 7.

Publications using NBP2-59770	Applications	Species
Vijayan M, Reddy PH Reduced VDAC1, Maintained Mitochondrial Dynamics and Enhanced Mitochondrial Biogenesis in a Transgenic Tau Mouse Model of Alzheimer's Disease International journal of molecular sciences 2022-08-02 [PMID: 35955694] (IF/IHC, WB, Mouse) Details: WB dilution used 1:1000, IHC dilution used 1:102	IF/IHC, WB	Mouse
Chen K, Chen C, Chen C et al. Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics Journal of Clinical Medicine 2021-12-27 [PMID: 35011880] (WB, Rat)	WB	Rat
Bhullar Ks, Shang N, Kerek E Et Al. Mitofusion is required for MOTS-c induced GLUT4 translocation Scientific reports 2021-07-12 [PMID: 34253808] (WB)	WB
Grieco J, Allen M, Perry J, et al. Progression-Mediated Changes in Mitochondrial Morphology Promotes Adaptation to Hypoxic Peritoneal Conditions in Serous Ovarian Cancer Frontiers in Oncology 2021-01-13 [PMID: 33520711] (WB, Mouse)	WB	Mouse
Gordillo GM, Biswas A, Singh K et al. Mitochondria as Target for Tumor Management of Hemangioendothelioma Antioxid. Redox Signal. 2020-07-28 [PMID: 32597200]
Ruegsegger GN, Manjunatha S, Summer P et al. Insulin deficiency and intranasal insulin alter brain mitochondrial function: a potential factor for dementia in diabetes. FASEB J. 2019-01-24 [PMID: 30676773] (WB, Mouse)	WB	Mouse
Nobis S, Goichon A, Achamrah N et al. Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia Sci Rep 2018-05-08 [PMID: 29740148] (WB, Mouse)	WB	Mouse

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Joshua Harvey	WB	Human	02/01/2021	View Summary Application Western Blot Sample Tested fibroblasts Species Human Lot CKXU0117051 Comments Comments SDS-PAGE run on a 8% Bis-Tris gel. Run in a biorad cassette system Loading gel - 100V then 120V to completion. Transfer 90minutes at 100V Blocked for 1 hour at RT with 5% Milk in PBS-T Primary: Stained overnight at 4oC with 1 in 1,000 OPA1 ab with 5% Milk in PBS-T Secondary: Rabbit anti-Goat. 1 in 30,000. Stained at RT for 1 hr with 5% Milk in PBS-T Loading control: 1 in 10,000 B-tubulin (Goat). Imaged using BioRad

Array NBP2-59770

• OPA1 Antibodies
• OPA1 Lysates
• OPA1 Proteins