![Western Blot: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Western-Blot-NBP2-73072-img0002.jpg "Western Blot: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI2E8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 81-210 human NT4 (NP_006170) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NTF4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 22.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Immunohistochemistry TrkB Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkB_AF1494_Immunohistochemistry_10167.jpg)
![Western Blot TrkB Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkB_AF1494_Western_Blot_23433.jpg)
![Western Blot TrkB Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkB_AF1494_Western_Blot_19007.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for NT-4 Antibody (NBP2-73072)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NTF4 |
![Immunocytochemistry/Immunofluorescence: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of A549 cells using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73072-img0003.jpg "Immunocytochemistry/Immunofluorescence: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of A549 cells using anti-NTF4 mouse monoclonal antibody.")
![Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Human colon tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73072-img0008.jpg "Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Human colon tissue using anti-NTF4 mouse monoclonal antibody.")
![Western Blot: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF4.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Western-Blot-NBP2-73072-img0001.jpg "Western Blot: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF4.")
![Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73072-img0004.jpg "Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NTF4 mouse monoclonal antibody.")
![Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73072-img0005.jpg "Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NTF4 mouse monoclonal antibody.")
![Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73072-img0006.jpg "Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NTF4 mouse monoclonal antibody.")
![Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73072-img0007.jpg "Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NTF4 mouse monoclonal antibody.")
![Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Human Kidney tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-OTI2E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73072-img0009.jpg "Immunohistochemistry: NT-4 Antibody (OTI2E8) - Azide and BSA Free [NBP2-73072] - Staining of paraffin-embedded Human Kidney tissue using anti-NTF4 mouse monoclonal antibody.")
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![SDS-PAGE beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_321.jpg)
![Bioactivity beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_322.jpg)
![Immunohistochemistry TrkA Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkA_AF1056_Immunohistochemistry_6672.jpg)
![Western Blot TrkA Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkA_AF1056_Western_Blot_17300.jpg)
![Immunohistochemistry TrkA Antibody [Unconjugated]](https://images.novusbio.com/images/af1056_rat-trka-affinity-purified-polyclonal-ab-8220249503264.jpg)
![Immunohistochemistry NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]](https://images.novusbio.com/images2/NGF_R_AF1157_Immunohistochemistry_6685.jpg)
![Western Blot NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]](https://images.novusbio.com/images2/NGF_R_AF1157_Western_Blot_17941.jpg)
![Western Blot TrkC Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkC_AF1404_Western_Blot_19346.jpg)
![Simple Western TrkC Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkC_AF1404_Simple_Western_20169.jpg)
![Immunocytochemistry/ Immunofluorescence TrkC Antibody [Unconjugated]](https://images.novusbio.com/images/af1404_mouse-rat-trkc-affinity-purified-polyclonal-ab-8220249503256.jpg)
![SDS-PAGE CNTF [Unconjugated]](https://images.novusbio.com/images2/CNTF_257-NT_502.jpg)
![Bioactivity CNTF [Unconjugated]](https://images.novusbio.com/images2/CNTF_257-NT_520.jpg)
![Bioactivity GDNF [Unconjugated]](https://images.novusbio.com/images2/GDNF_212GD_2846.jpg)
![SDS-PAGE GDNF [Unconjugated]](https://images.novusbio.com/images2/GDNF_212-GD_62.jpg)
![SEC-MALS GDNF [Unconjugated]](https://images.novusbio.com/images/212-gd_recombinant-human-gdnf-protein-sec-mals-25202393740..jpg)
![Neutralization CNTFR alpha Antibody [Unconjugated]](https://images.novusbio.com/images2/CNTF_R_alpha_AF-303-NA_Block_Neutralize_763.jpg)
![Western Blot LEDGF Antibody (762815) [Unconjugated]](https://images.novusbio.com/images2/12543.jpg)
![Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]](https://images.novusbio.com/images2/12613.jpg)
![Immunohistochemistry Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Immunohistochemistry_7161.jpg)
![Western Blot Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Western_Blot_19100.jpg)
![Simple Western Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Simple_Western_20098.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/TNF_RII_DRT200_ELISA_176.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/DATA_TNF_RII_DRT200_ELISA_817.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/DATA_TNF_RII_DRT200_ELISA_818.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")