Novus Biologicals products are now on bio-techne.com

NT-4 Antibody (OTI2E8)

Images

 
Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.
Immunocytochemistry/ Immunofluorescence: NT-4 Antibody (2E8) [NBP1-47897] - Staining of A549 cells using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human Kidney tissue using anti-NTF4 mouse monoclonal antibody.
Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human colon tissue using anti-NTF4 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

NT-4 Antibody (OTI2E8) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 81-210 human NT4 (NP_006170) produced in E.coli.
Specificity
This antibody is specific for Homo sapiens neurotrophin 4 (NTF4).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NTF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:5000
Theoretical MW
22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-47897.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Please contact Technical Support if you have any Mouse-On-Mouse questions. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NT-4 Antibody (OTI2E8)

  • GLC10
  • GLC1O
  • neurotrophic factor 4
  • neurotrophic factor 5
  • neurotrophin 4
  • neurotrophin 5 (neurotrophin 4/5)
  • neurotrophin-4
  • neurotrophin-5
  • Neutrophic factor 4
  • NT4
  • NT-4
  • NT-4/5
  • NT-5
  • NTF4
  • NTF5
  • NTF5NT5

Background

NT4 is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of NT4 is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NT4-deficient mice only show minor cellular deficits and develop normally to adulthood

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

DBD00
Species: Hu
Applications: ELISA
267-N3
Species: Hu
Applications: BA
256-GF
Species: Hu
Applications: BA
AF1494
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB
AF1056
Species: Rt
Applications: IHC, WB
AF1157
Species: Mu
Applications: IHC, WB
AF1404
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
H00007170-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
257-NT
Species: Hu
Applications: BA
212-GD
Species: Hu
Applications: Bind, BA
AF-303-NA
Species: Hu
Applications: Neut, WB
MAB3468
Species: Hu
Applications: ICC, WB
NBP2-33903
Species: Hu
Applications: IHC, IHC-P
AF2934
Species: Mu
Applications: Block, IHC, Simple Western, WB
DRT200
Species: Hu
Applications: ELISA
NBP1-18910
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
H00003059-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
MAB224
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
H00001523-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP1-47897
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for NT-4 Antibody (NBP1-47897)(1)

Reviews for NT-4 Antibody (NBP1-47897) (0)

There are no reviews for NT-4 Antibody (NBP1-47897). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NT-4 Antibody (NBP1-47897) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NT-4 Antibody (OTI2E8) and receive a gift card or discount.

Bioinformatics

Gene Symbol NTF4
Entrez