![Immunohistochemistry-Frozen: Notch3 Antibody [NB100-93550] - Notch 3 homolog staining was performed on 10 micron crysostat sections of rat brain fixed with 4% formaldehyde by transcardial perfusion. 1:250 dilution of Notch 3 antibody was employed and incubation was performed overnight at 4C. Stained tissues were mounted under iBright mounting media.](http://images.novusbio.com/fullsize/Notch3-Antibody-Immunohistochemistry-Frozen-NB100-93550-img0002.jpg "Immunohistochemistry-Frozen: Notch3 Antibody [NB100-93550] - Notch 3 homolog staining was performed on 10 micron crysostat sections of rat brain fixed with 4% formaldehyde by transcardial perfusion. 1:250 dilution of Notch 3 antibody was employed and incubation was performed overnight at 4C. Stained tissues were mounted under iBright mounting media.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide representing the C terminal region of the human NOTCH homolog 3 (NOTCH3) protein. |
| Specificity | Notch 3 homolog |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NOTCH3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This Notch3 antibody is useful for Western Blot, ELISA and immunohistochemistry-Frozen applications. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 26341090). |
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| Theoretical MW | 243.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-93550 | Applications | Species |
|---|---|---|
| Soylu H, Acar N, Ozbey O et al. Characterization of Notch Signalling Pathway Members in Normal Prostate, Prostatic Intraepithelial Neoplasia (PIN) and Prostatic Adenocarcinoma. Pathol Oncol Res 2016-01-01 [PMID: 26341090] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Notch-3 Antibody (NB100-93550)Find related products by research area.
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