Nestin Antibody (4D11)

Nestin (NES) is a class VI intermediate filament protein which is unable to self-assemble or form homodimers because of a short NH2-terminus and is essential for the development of brain, eye etc. as well as for processes such as survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. It facilitates the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and the trafficking/distribution of IF proteins or other factors to daughter cells during progenitor cell division. Nestin is expressed in dividing cells during early stages of development e.g. CNS, PNS, myogenic or other tissues etc, whereas with differentiation, it is downregulated followed by replacement with tissue-specific intermediate filament proteins, and therefore, is widely used as a neuronal stem cell marker. Under pathological conditions, nestin is expressed in repair processes in CNS, muscle, liver, and infarcted myocardium, and in tumors, increased nestin correlates with aggressive growth, metastasis, and poor prognosis. Furthermore, nestin is more specifically expressed in proliferating small-sized tumor vessels in glioblastoma and gastric, colorectal, and prostate cancers than are other tumor vessel markers, indicating nestin's use as a marker for newly synthesized tumor vessels.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC, ICC/IF, IF, IF/IHC, IHC.

Showing Publications 1 - 10 of 24. Show All 24 Publications. Collapse Publications.

Publications using NBP1-92717	Applications	Species
Chang SY, Kim E, Carpena NT et al. Photobiomodulation Can Enhance Stem Cell Viability in Cochlea with Auditory Neuropathy but Does Not Restore Hearing Stem cells international 2023-11-15 [PMID: 38020205] (IHC)	IHC
Tan R, Hu X, Wang X et al. Leptin Promotes the Proliferation and Neuronal Differentiation of Neural Stem Cells through the Cooperative Action of MAPK/ERK1/2, JAK2/STAT3 and PI3K/AKT Signaling Pathways International journal of molecular sciences 2023-10-13 [PMID: 37894835] (ICC/IF, Rat)	ICC/IF	Rat
Chen L, Wang J, Yang T et al. Establishment of iPS cell line (KLRMMEi003-A) from a patient with Usher syndrome due to USH2A mutation Stem cell research 2023-02-28 [PMID: 36863132] (ICC/IF, Human) Details: Dilution used in ICC/IF 1:200	ICC/IF	Human
Huang LY, Ma JY, Song JX et al. Ischemic accumulation of succinate induces Cdc42 succinylation and inhibits neural stem cell proliferation after cerebral ischemia/reperfusion Neural regeneration research 2023-05-01 [PMID: 36254990] (ICC/IF, Mouse)	ICC/IF	Mouse
Sanjurjo-Soriano C, Erkilic N, Vache C et al. Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa Stem Cell Research 2022-04-01 [PMID: 35248879] (IF/IHC, Human)	IF/IHC	Human
Liang L, Xue Y, Su C et al. Establishment of iPS cell line (KLRMMEi002-A) by reprogramming peripheral blood mononuclear cells from a patient with USH2A-associated Usher syndrome Stem Cell Res 2022-02-13 [PMID: 35152177]
Goranci-Buzhala G, Mariappan A, Ricci-Vitiani L et al. Cilium induction triggers differentiation of glioma stem cells Cell reports 2021-09-07 [PMID: 34496239] (ICC/IF, Human)	ICC/IF	Human
Mifsud KR, Kennedy CLM, Salatino S Et al. Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors Nature communications 2021-08-06 [PMID: 34362910] (ICC/IF, Human)	ICC/IF	Human
Abdalla Y, Luo M, MAkilA E et al. Effectiveness of porous silicon nanoparticle treatment at inhibiting the migration of a heterogeneous glioma cell population Journal of nanobiotechnology 2021-02-26 [PMID: 33637089] (ICC/IF, Human)	ICC/IF	Human
Guo X, Wang D, Shan X et al. Establishment of an induced pluripotent stem cell line from a Antley-Bixler Syndrome (ABS) patient with the homozygote mutation p.R457H (c.1370G>A) in POR gene Stem cell research 2021-02-18 [PMID: 33631521] (ICC, Human)	ICC	Human
Ji W, Wang D, Chen C et al. Generation of an induced pluripotent stem cell line from a Bartter syndrome patient with the homozygote mutation p.A244D (c.731>A) in SLC12A1 gene Stem cell research 2021-02-10 [PMID: 33607471] (ICC/IF, Human)	ICC/IF	Human
Chen H, Ding Y, Zhou W, et al. Establishment of an induced pluripotent stem cell line (WMUi016-A) from a patient with X-linked Dent disease (X-Dent) carrying the hemizygote mutation p.R718* (c.2152 C>T) in the CLCN5 gene Stem Cell Research 2021-01-01 [PMID: 33545640] (ICC/IF, FLOW, Human)	ICC/IF, FLOW	Human
Guo X, Zhang X, Wang L, et al. Generation of an urine-derived induced pluripotent stem cell line WMUi017-A from a X-linked Renpenning syndrome (X-RSY) patient with the hemizygous PQBP1 gene mutation p.P609A (c.1825C>G) Stem cell research 2021-01-08 [PMID: 33477035] (ICC/IF, Human)	ICC/IF	Human
Guo X, Ji W, Niu C et al. Generation of an urine-derived induced pluripotent stem cell line from a 5-year old X-linked Alport syndrome (X-LAS) patient Stem Cell Res 2020-11-08 [PMID: 33202306] (FLOW, ICC/IF)	FLOW, ICC/IF
Guo X, Wang L, Chen K et al. Generation of urine-derived iPS cell line via a non-integrative method from a Barth syndrome patient with TAZ gene mutation Stem Cell Research 2020-01-01 [PMID: 32619718] (ICC/IF, Human)	ICC/IF	Human
Erkilic N, Sanjurjo-Soriano C, Manes G et al. Generation of a human iPSC line, INMi004-A, with a point mutation in CRX associated with autosomal dominant Leber congenital amaurosis Stem Cell Res 2019-06-03 [PMID: 31247521] (IF, Human)	IF	Human
Erkilic N, Sanjurjo-Soriano C, Diakatou M et al. Generation of a human iPSC line, INMi003-A, with a missense mutation in CRX associated with autosomal dominant cone-rod dystrophy Stem Cell Res 2019-06-07 [PMID: 31203166] (FLOW, Human)	FLOW	Human
Goulielmaki M, Assimomytis N, Rozanc J et al. Establishment of CSUASOi001-A, a non-integrated induced pluripotent stem cell line from urine-derived cells of a Chinese patient carrying RS1 gene mutation Stem Cell Res 2019-05-17 [PMID: 31141763] (ICC/IF, Human)	ICC/IF	Human
Guo Y, Zeng Q, Liu S et al. Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa. Stem Cell Res 2018-04-06 [PMID: 29660607] (ICC/IF, Human)	ICC/IF	Human
Gabriel E, Gopalakrishnan J. Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders. J Vis Exp. 2017-04-14 [PMID: 28448044]
Gabriel E, Ramani A, Karow U et al. Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids Cell Stem Cell 2017-01-23 [PMID: 28132835] (ICC/IF, Human)	ICC/IF	Human
Moncla BJ, Chappell CA, Debo BM, Meyn LA. The Effects of Hormones and Vaginal Microflora on the Glycome of the Female Genital Tract: Cervical-Vaginal Fluid PLoS ONE 2016-07-21 [PMID: 27437931] (ICC/IF, Human)	ICC/IF	Human
Hockfield S, McKay RD. Identification of major cell classes in the developing mammalian nervous system. J Neurosci. 1985-12-01 [PMID: 4078630]
Lendahl U, Zimmerman LB, McKay RD et al. CNS stem cells express a new class of intermediate filament protein. Cell. 1990-02-01 [PMID: 1689217]
Show All 24 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	Immunohistochemistry-microslice section	Mouse	07/27/2017	View Summary Application Other Sample Tested Mouse cortex Species Mouse Lot 6315

Array NBP1-92717

• Nestin Antibodies
• Nestin Proteins