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Nestin Antibody (10C2) [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rb(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
10C2
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

Nestin Antibody (10C2) [HRP] Summary

Immunogen
A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System

Reactivity Notes

This antibody does not cross-react with rat or rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Nestin Antibody (10C2) [HRP]

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (NB300-266H) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB300-266H). (Showing 1 - 1 of 1 FAQ).

  1. What is the retrieval pH for nestin NB300-266 and Alpha Lactalbumin NB100-2783 used by the company?
    • We usually recommend using citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieval. NB100-2783 has only been validated for IF and western blot but if you would like to use it for IHC, you are more than welcome to do so. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Secondary Antibodies

 

Isotype Controls

Additional Nestin Products

Research Areas for Nestin Antibody (NB300-266H)

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Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Product PDFs

Calculators

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Bioinformatics

Gene Symbol NES