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NCK1 Antibody (5B7) - BSA Free

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Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.
Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.
Immunohistochemistry: NCK1 Antibody (5B7) [NBP2-37490] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.
Flow Cytometry: NCK1 Antibody (5B7) [NBP2-37490] - Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).
Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa)
ELISA: NCK1 Antibody (5B7) [NBP2-37490] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Pm, PmSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
5B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37490-0.025ml
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NCK1 Antibody (5B7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human NCK1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCK1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for NCK1 Antibody (5B7) - BSA Free

  • cytoplasmic protein NCK1
  • MGC12668
  • NCK adaptor protein 1melanoma NCK protein
  • NCK1
  • nck-1
  • NCKalpha
  • NCKNCK tyrosine kinase
  • non-catalytic region of tyrosine kinase
  • SH2/SH3 adaptor protein NCK-alpha

Background

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NCK1