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NANS Antibody

Images

 
Western Blot: NANS Antibody [NBP1-31440] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE NANS antibody, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: NANS Antibody [NBP1-31440] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NANS protein stained by NANS antibody diluted at 1:500.Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: NANS Antibody [NBP1-31440] - Paraffin-embedded normal gastric epithelium (gland), using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NANS Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NANS. The exact sequence is proprietary.
Predicted Species
Mouse (97%), Rat (97%), Porcine (97%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NANS
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for NANS Antibody

  • EC 2.5.1.56
  • EC 2.5.1.57
  • N-acetylneuraminate synthase
  • N-acetylneuraminate-9-phosphate synthase
  • N-acetylneuraminic acid phosphate synthase
  • N-acetylneuraminic acid synthasesialic acid phosphate synthase
  • SASsialic acid synthase

Background

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NANS Antibody (NBP1-31440) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on NANS

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Bioinformatics

Gene Symbol NANS