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NANS Antibody (3G6)

Images

 
Western Blot: NANS Antibody (3G6) [H00054187-M01] - NANS monoclonal antibody (M01), clone 3G6 Analysis of NANS expression in HeLa.
Sandwich ELISA: NANS Antibody (3G6) [H00054187-M01] - Detection limit for recombinant GST tagged NANS is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NANS Antibody (3G6) Summary

Immunogen
NANS (NP_061819, 260 a.a. ~ 359 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AELVRSVRLVERALGSPTKQLLPCEMACNEKLGKSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPEDIFNLVGKKVLVTVEEDDTIMEELVDNHGKKIKS
Specificity
NANS - N-acetylneuraminic acid synthase (sialic acid synthase)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NANS
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Publications
Read Publication using H00054187-M01.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NANS Antibody (3G6)

  • EC 2.5.1.56
  • EC 2.5.1.57
  • N-acetylneuraminate synthase
  • N-acetylneuraminate-9-phosphate synthase
  • N-acetylneuraminic acid phosphate synthase
  • N-acetylneuraminic acid synthasesialic acid phosphate synthase
  • SASsialic acid synthase

Background

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NANS Antibody (H00054187-M01)(1)


Showing Publication 1 - 1 of 1.
Publication using H00054187-M01 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NANS
Entrez
OMIM
Uniprot