![Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Azide-and-BSA-Free-Immunohistochemistry-Frozen-NBP2-33148-img0007.jpg "Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.")
| Reactivity | Hu, Eq, Rt(-)Species Glossary |
| Applications | WB, Flow, IHC |
| Clone | M2-7C10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15197). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant Melan-A/MART-1 protein (Uniprot: Q16655) |
| Localization | Cytoplasmic |
| Marker | Melanoma Marker |
| Specificity | This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. |
| Isotype | IgG2b Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLANA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Melan-A/MART-1 Antibody (NBP2-33148)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33148-img0003.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33148-img0004.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-fixed, paraffin-embedded human skin stained with MART-1 Monoclonal Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33148-img0005.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-fixed, paraffin-embedded human skin stained with MART-1 Monoclonal Antibody (M2-7C10).")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33148-img0006.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) - Azide and BSA Free [NBP2-33148] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.")
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
