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MBP Antibody (932908) [Unconjugated]

 (11 Publications)
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Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by ...read more
Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) ...read more
MBP was detected in immersion fixed rat cortical stem cells differentiated for 7 days to oligodendrocytes using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 10 µg/mL for 3 hours at room ...read more
MBP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 1.7 µg/mL for 1 hour at room temperature ...read more
MBP was detected in perfusion fixed frozen sections of mouse brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
MBP was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
932908
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB42282-SP
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MBP Antibody (932908) [Unconjugated] Summary

Immunogen
Purified MBP from bovine brain
Accession # P02686
Specificity
Detects human, mouse, and rat MBP
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MBP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.1-25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB42282 in the following applications:

  • IHC
    4 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MBP Antibody (932908) [Unconjugated]

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Background

Myelin Basic Protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBP Antibody (MAB42282)(11)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(4)
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Filter By Species
Mouse
(1)
Rat
(2)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB42282 Applications Species
Lee JY, Harney DJ, Teo JD et al. The major TMEM106B dementia risk allele affects TMEM106B protein levels, fibril formation, and myelin lipid homeostasis in the ageing human hippocampus Mol Neurodegener 2023-09-19 [PMID: 37726834]
Lee JY, Harney DJ, Teo JD et al. The major TMEM106B dementia risk allele affects TMEM106B protein levels, fibril formation, and myelin lipid homeostasis in the ageing human hippocampus Mol Neurodegener 9/19/2023 [PMID: 37726834]
Yu-Lung Lin, Jennifer Nhieu, Thomas Lerdall, Liming Milbauer, Chin-Wen Wei, Dong Jun Lee et al. A novel 3D bilayer hydrogel tri-culture system for studying functional motor units Cell Biosci 9/12/2023 [PMID: 37700376]
You Y, Muraoka S, Jedrychowski MP et al. Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain Journal of Extracellular Vesicles 1/13/2022 [PMID: 35029059]
You Y, Muraoka S, Jedrychowski MP et al. Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain Journal of Extracellular Vesicles 2022-01-13 [PMID: 35029059]
Y Wu, W Shao, TW Todd, J Tong, M Yue, S Koga, M Castanedes, AL Librero, CW Lee, IR Mackenzie, DW Dickson, YJ Zhang, L Petrucelli, M Prudencio Microglial lysosome dysfunction contributes to white matter pathology and TDP-43 proteinopathy in GRN-associated FTD Cell Reports, 2021-08-24;36(8):109581. 2021-08-24 [PMID: 34433069] (IHC, Mouse) IHC Mouse
Q Zhang, W Song, B Zhao, J Xie, Q Sun, X Shi, B Yan, G Tian, X Liang Quercetin Attenuates Diabetic Peripheral Neuropathy by Correcting Mitochondrial Abnormality via Activation of AMPK/PGC-1&alpha Pathway in vivo and in vitro Frontiers in Neuroscience, 2021-03-03;15(0):636172. 2021-03-03 [PMID: 33746703] (IHC, Rat) IHC Rat
Y Tanaka, N Nakagomi, N Doe, A Nakano-Doi, T Sawano, T Takagi, T Matsuyama, S Yoshimura, T Nakagomi Early Reperfusion Following Ischemic Stroke Provides Beneficial Effects, Even After Lethal Ischemia with Mature Neural Cell Death Cells, 2020-06-01;9(6):. 2020-06-01 [PMID: 32492968] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Tyler C. Huff, David W. Sant, Vladimir Camarena, Derek Van Booven, Nadja S. Andrade, Sushmita Mustafi et al. Vitamin C regulates Schwann cell myelination by promoting DNA demethylation of pro‐myelinating genes Journal of Neurochemistry 6/1/2021 [PMID: 32219848]
Justyna Ulanska-Poutanen, Jakub Mieczkowski, Chao Zhao, Katarzyna Konarzewska, Beata Kaza, Hartmut BF Pohl et al. Injury-induced perivascular niche supports alternative differentiation of adult rodent CNS progenitor cells eLife 9/17/2018 [PMID: 30222103]
Show All 11 Publications.

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Bioinformatics

Gene Symbol MBP
Uniprot