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JAZF1 Antibody (PCRP-JAZF1-1C2)

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Immunocytochemistry/ Immunofluorescence: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885] - Immunofluorescence analysis of PFA-fixed HeLa cells. JAZF1 Antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (green). ...read more
Immunohistochemistry-Paraffin: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885] - Formalin-fixed, paraffin-embedded human testis stained. Strong nuclear staining using JAZF1 antibody (PCRP-JAZF1-1C2) at 2ug/ml. HIER: ...read more
Flow Cytometry: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885] - Flow cytometric analysis of PFA-fixed HeLa cells. JAZF1 antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Protein Array: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885] - Analysis of Protein Array containing more than 19,000 full-length human proteins using JAZF1 Antibody (PCRP-JAZF1-1C2).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MA
Clone
PCRP-JAZF1-1C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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JAZF1 Antibody (PCRP-JAZF1-1C2) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14221)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary) (Uniprot: Q86VZ6)
Localization
Nucleus
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
JAZF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Theoretical MW
27.08 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for JAZF1 Antibody (PCRP-JAZF1-1C2)

  • JAZF zinc finger 1
  • juxtaposed with another zinc finger gene 1
  • juxtaposed with another zinc finger protein 1
  • TAK1-interacting protein 27
  • TIP27ZNF802DKFZp761K2222
  • Zinc finger protein 802

Background

The JAZF1 gene encodes a nuclear protein with three C2H2-type zinc fingers, and functions as a transcriptional repressor.Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternatively splicedvariants which encode diff

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for JAZF1 Antibody (NBP3-13885) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol JAZF1
Uniprot