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CAMKID Antibody

Images

 
Western Blot: CAMKID Antibody [NBP1-85589] - Analysis in control (vector only transfected HEK293T lysate) and CAMK1D over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: CAMKID Antibody [NBP1-85589] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CAMKID Antibody [NBP1-85589] - Staining of human liver shows no positivity in hepatocytes as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: CAMKID Antibody [NBP1-85589] - Analysis in human cerebral cortex and liver tissues. Corresponding CAMK1D RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CAMKID Antibody [NBP1-85589] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in endothelial cells.
Immunohistochemistry-Paraffin: CAMKID Antibody [NBP1-85589] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: CAMKID Antibody [NBP1-85589] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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CAMKID Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGG
Predicted Species
Mouse (100%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK1D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CAMKID Protein (NBP1-85589PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CAMKID Antibody

  • calcium/calmodulin-dependent protein kinase ID
  • calcium/calmodulin-dependent protein kinase type 1D
  • CaM kinase I delta
  • CaM kinase ID
  • CaM-K1
  • CaMKI delta
  • CaM-KI delta
  • CaMKID
  • CaMKI-like protein kinase
  • CKLiKEC 2.7.11.17
  • EC 2.7.11

Background

CAMK1D encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. The encoded protein may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CAMKID Products

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Bioinformatics

Gene Symbol CAMK1D