Home » INTS1 » INTS1 Lysates » INTS1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

INTS1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: INTS1 Overexpression Lysate (Adult Normal) [NBP2-08335] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for INTS1.
Western Blot: INTS1 Overexpression Lysate (Adult Normal) [NBP2-08335] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for INTS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

INTS1 Overexpression Lysate Summary

Description

INTS1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC217456

Accession#: NM_001080453

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
INTS1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
244.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for INTS1 Overexpression Lysate

  • DKFZp586J0619
  • FLJ46624
  • integrator complex subunit 1
  • NET28
  • UNQ1821/PRO3434

Background

INTS1 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

FGF-3
[Unconjugated]
1206-F3
FGF-3 [Unconjugated]
(13 Publications)
Species: Hu
Applications: BA
GLI-1 Antibody -
BSA Free
NB600-600
Immunohistochemistry-Paraffin GLI-1 Antibody - BSA Free
Knockdown Validated GLI-1 Antibody - BSA Free
Flow (Intracellular) GLI-1 Antibody - BSA Free
(1 Review)
(55 Publications)
Species: Ca, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, IB, ICC/IF, IHC, IHC-P, KD, WB
INT3 Antibody
NBP2-57633
Immunohistochemistry-Paraffin INT3 Antibody
Immunocytochemistry/ Immunofluorescence INT3 Antibody
Immunohistochemistry-Paraffin INT3 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Wnt-1 Antibody
(10C8) - BSA Free
NBP1-51575
Immunocytochemistry/ Immunofluorescence Wnt-1 Antibody (10C8) - BSA Free
Immunohistochemistry-Paraffin Wnt-1 Antibody (10C8) - BSA Free
Western Blot Wnt-1 Antibody (10C8) - BSA Free
(2 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Wnt3 Antibody
(OTI1G7)
NBP2-46367
Immunohistochemistry Wnt3 Antibody (OTI1G7)
Immunohistochemistry Wnt3 Antibody (OTI1G7)
Immunohistochemistry Wnt3 Antibody (OTI1G7)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
LRP-5 Antibody
AF6646
Western Blot LRP-5 Antibody
Species: Hu
Applications: WB
Notch-4 Antibody
[Unconjugated]
AF3847
Immunocytochemistry Notch-4 Antibody [Unconjugated]
(2 Publications)
Species: Hu
Applications: ICC, WB
CNOT8 Antibody
NBP2-15930
Western Blot CNOT8 Antibody
Immunocytochemistry/ Immunofluorescence CNOT8 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, WB
Phenylalanine
Hydroxylase Antibody
NBP2-48615
Immunohistochemistry-Paraffin Phenylalanine Hydroxylase Antibody
Immunohistochemistry-Paraffin Phenylalanine Hydroxylase Antibody
Immunohistochemistry-Paraffin Phenylalanine Hydroxylase Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(445 Publications)
Species: Hu
Applications: BA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
INT4 Antibody
NB100-60660
Western Blot INT4 Antibody
Immunohistochemistry-Paraffin INT4 Antibody
Immunohistochemistry-Paraffin INT4 Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
alpha
2-Macroglobulin Antib ...
AF1938
Simple Western alpha 2-Macroglobulin Antibody [Unconjugated]
Western Blot alpha 2-Macroglobulin Antibody [Unconjugated]
Immunocytochemistry alpha 2-Macroglobulin Antibody [Unconjugated]
(4 Publications)
Species: Hu
Applications: ICC, IP, Simple Western, WB
TLR4 Antibody
(76B357.1) - BSA Free
NB100-56566
Dual RNAscope ISH-IHC TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
(5 Reviews)
(140 Publications)
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
HOXC4 Antibody
NBP2-56195
Western Blot HOXC4 Antibody
Immunocytochemistry/ Immunofluorescence HOXC4 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, WB
HOXC5 Antibody
(1E10)
H00003222-M01
Sandwich ELISA HOXC5 Antibody (1E10)
(2 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, S-ELISA, WB
HOXC6 Antibody
(2A4)
H00003223-M04
Western Blot HOXC6 Antibody (2A4)
ELISA HOXC6 Antibody (2A4)
(1 Publication)
Species: Hu
Applications: ELISA, WB
INTS1
Overexpression Lysate
NBP2-08335
INTS1 Overexpression Lysate
Species: Hu
Applications: WB

Publications for INTS1 Lysate (NBP2-08335) (0)

There are no publications for INTS1 Lysate (NBP2-08335).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for INTS1 Lysate (NBP2-08335) (0)

There are no reviews for INTS1 Lysate (NBP2-08335). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for INTS1 Lysate (NBP2-08335) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional INTS1 Products

Blogs on INTS1

There are no specific blogs for INTS1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma .
IFN-gamma [Unconjugated]
285-IF-100
Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.
TLR4 Antibody (76B357.1) - BSA Free
NB100-56566

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our INTS1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol INTS1