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IL-10 R beta Antibody [Unconjugated]

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A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL-10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5368, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL-10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5368, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF5368
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Catalog# & Formulation Size Price
Carrier Free
AF5368-SP
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IL-10 R beta Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-10 R beta
Ile21-Ser220
Accession # Q61190
Specificity
Detects mouse IL-10 R beta in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) IL‑10 R beta is observed and less than 1% cross-reactivity with rhIL-10 R and recombinant mouse IL-10 R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL10RB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publication using
AF5368 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 R beta Antibody [Unconjugated]

  • CDw210b antigen
  • CDw210b
  • CRF2-4IL-10R subunit 2
  • CRFB4
  • CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B
  • D21S58
  • D21S66
  • IBD25
  • IL-10 R beta
  • IL-10 receptor subunit beta
  • IL10R beta
  • IL-10R2
  • IL-10R2IL-10R subunit beta
  • IL10RB
  • IL-10Rb
  • interleukin 10 receptor, beta
  • Interleukin-10 receptor subunit 2
  • interleukin-10 receptor subunit beta
  • member 4

Background

IL-10 R beta (Interleukin 10 receptor beta; also IL‑10 R2, CD210b and CRF2-4) is a 60‑65 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed, and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein that is 330 amino acids (aa) in length. It contains a 201 aa extracellular domain (aa 20‑220) that possesses two fibronectin type III domains (aa 22‑107 and 111‑208). There is one alternate start site at Met20. Over aa 21‑220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 R beta Antibody (AF5368)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol IL10RB
Uniprot