Home » IL-22BP/IL22 RA2 » IL-22BP/IL22 RA2 Proteins » Recombinant Human IL-22BP His-tag Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Human IL-22BP His-tag Protein

 Submit a Review

Images

 
There are currently no images for IL-22BP/IL22 RA2 (8498-BP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
8498-BP-025/CF
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human IL-22BP His-tag Protein Summary

Details of Functionality
Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is typically 1-5 ng/mL in the presence of 1 ng/mL of Recombinant Human IL-22 (Catalog # 782-IL).
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-22BP protein
Thr22-Pro231, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Thr22
Protein/Peptide Type
Recombinant Proteins
Gene
IL22RA2
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
46-53 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-22BP His-tag Protein

  • class II cytokine receptor
  • CRF2-10
  • CRF2-S1IL22BP
  • CRF2-X
  • Cytokine receptor class-II member 10
  • Cytokine receptor family 2 member 10
  • Cytokine receptor family type 2, soluble 1
  • IL-22 RA2
  • IL-22 receptor subunit alpha-2
  • IL22BP
  • IL-22BP
  • IL-22BPCRF2X
  • IL22RA2
  • IL-22RA2
  • IL-22R-alpha-2
  • interleukin 22 receptor, alpha 2
  • interleukin 22-binding protein
  • interleukin-22 receptor subunit alpha-2
  • Interleukin-22-binding protein
  • MGC150509
  • MGC150510
  • zcytoR16

Background

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta . IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1, 2). IL-22BP blocks the interaction of IL-22 with IL-22 R, preventing IL-22 induced production of reactive oxygen species, IL-6, IL-10, and TNF-a (3-8). In vivo, it regulates the proinflammatory effects of IL-22 (e.g. neutrophil infiltration) but not of IL-10 (7). Mouse IL-22BP can neutralize the bioactivity of both mouse and human IL-22 (6). IL-22BP is produced by dendritic cells (DC), epithelial cells, activated B cells, and activated monocytes (3, 6, 9, 10). It is constitutively expressed by DC but is down-regulated during local inflammation and in response to tissue damage (11-13). IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). Mature human IL-22BP contains two Fibronectin type III domains (3, 4). Alternative splicing generates additional isoforms that contain a 32 amino acid (aa) insertion in the first Fn-III domain and may also be truncated within the second Fn-III domain (3, 4, 14). Human IL-22BP without the 32 aa insertion shares 68% and 73% amino acid (aa) sequence identity with mouse and rat IL-22BP, respectively.
  1. Lim, C. and R. Savan (2014) Cytokine Growth Factor Rev. 25:257.
  2. Mizoguchi, A. (2012) Inflamm. Bowel Dis. 18:1777.
  3. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
  4. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  5. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  6. Wei, C.-C. et al. (2003) Genes Immun. 4:204.
  7. Weber, G.F. et al. (2007) Infect. Immun. 75:1690.
  8. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
  9. Lecart, S. et al. (2002) Int. Immunol. 14:1351.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Wolk, K. et al. (2007) J. Immunol. 178:5973.
  12. Huber, S. et al. (2012) Nature 491:259.
  13. Martin, J.C.J. et al. (2014) Mucosal Immunol. 7:101.
  14. Dumoutier, L. et al. (2001) J. Immunol. 166:7090.

Customers Who Viewed This Item Also Viewed...

IL-22
[Unconjugated]
782-IL/CF
SDS-PAGE IL-22 [Unconjugated]
Bioactivity IL-22 [Unconjugated]
(65 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(295 Publications)
Species: Mu
Applications: ELISA
IL-17D Antibody
(246018) [Unconjugate
MAB15041
Immunohistochemistry IL-17D Antibody (246018) [Unconjugated]
Intracellular Staining by Flow Cytometry IL-17D Antibody (246018) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
IL-10 R beta
Antibody (90220) [Unc ...
MAB874
Neutralization IL-10 R beta Antibody (90220) [Unconjugated]
Flow Cytometry IL-10 R beta Antibody (90220) [Unconjugated]
(1 Review)
(9 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, Neut, WB
IL-17B Antibody
[Unconjugated]
AF1709
Intracellular Staining by Flow Cytometry IL-17B Antibody [Unconjugated]
(2 Publications)
Species: Mu
Applications: Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICFlow, WB
IL-20
[Unconjugated]
1102-IL/CF
IL-20 [Unconjugated]
(11 Publications)
Species: Hu
Applications: BA
IL-26/AK155
Antibody
AF1375
IL-26/AK155 Antibody
(6 Publications)
Species: Hu
Applications: WB
IL-22R alpha 1
Antibody (305405) [Un ...
MAB2770
Flow Cytometry IL-22R alpha 1 Antibody (305405) [Unconjugated]
Immunocytochemistry IL-22R alpha 1 Antibody (305405) [Unconjugated]
Immunocytochemistry IL-22R alpha 1 Antibody (305405) [Unconjugated]
(11 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC
IL-17F [Biotin]
DY1335B
N/A IL-17F [Biotin]
N/A IL-17F [Biotin]
(13 Publications)
Species: Hu
Applications: ELISA
IL-19 [HRP]
D1900
N/A IL-19 [HRP]
N/A IL-19 [HRP]
(7 Publications)
Species: Hu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(450 Publications)
Species: Hu
Applications: BA
STAT3 Antibody
(232209) [Unconjugate
MAB1799
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
Western Blot STAT3 Antibody (232209) [Unconjugated]
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
(22 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(809 Publications)
Species: Hu
Applications: BA
IL-20R alpha
Antibody (173707) [Un ...
MAB11761
Immunohistochemistry IL-20R alpha Antibody (173707) [Unconjugated]
(4 Publications)
Species: Hu
Applications: IHC
SOAT1 Antibody
NB400-141
SOAT1 Antibody
(7 Publications)
Species: Ha, Hu, Mu
Applications: ICC/IF, IP, WB
IL-24
[Unconjugated]
1965-IL
IL-24 [Unconjugated]
(19 Publications)
Species: Hu
Applications: BA
IL-9 [Unconjugated]
409-ML
IL-9 [Unconjugated]
(13 Publications)
Species: Mu
Applications: BA
IFN-gamma R1/CD119
Antibody (92101) [Unc ...
MAB6731
Neutralization IFN-gamma R1/CD119 Antibody (92101) [Unconjugated]
(13 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, Neut, WB

Publications for IL-22BP/IL22 RA2 (8498-BP) (0)

There are no publications for IL-22BP/IL22 RA2 (8498-BP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-22BP/IL22 RA2 (8498-BP) (0)

There are no reviews for IL-22BP/IL22 RA2 (8498-BP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-22BP/IL22 RA2 (8498-BP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-22BP/IL22 RA2 Products

Blogs on IL-22BP/IL22 RA2

There are no specific blogs for IL-22BP/IL22 RA2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IL-22BP His-tag Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol IL22RA2
Uniprot