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HGK/MAP4K4 Antibody

Images

 
Western Blot: HGK/MAP4K4 Antibody [NBP1-58853] - Sample Tissue: Human Stomach Tumor Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: HGK/MAP4K4 Antibody [NBP1-58853] - Liver
Western Blot: HGK/MAP4K4 Antibody [NBP1-58853] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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HGK/MAP4K4 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to MAP4K4(mitogen-activated protein kinase kinase kinase kinase 4) The peptide sequence was selected from the N terminal of MAP4K4. Peptide sequence PFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQ. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP4K4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Publications
Read Publication using
NBP1-58853 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for HGK/MAP4K4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • FLH21957
  • FLJ10410
  • hepatocyte progenitor kinase-like/germinal center kinase-like kinase
  • HGK
  • HGKFLJ20373
  • HPK/GCK-like kinase HGK
  • KIAA0687
  • MAP4K4
  • MAPK/ERK kinase kinase kinase 4
  • MEK kinase kinase 4
  • MEKKK 4
  • MEKKK4
  • mitogen-activated protein kinase kinase kinase kinase 4
  • Nck-interacting kinase
  • NIKFLJ90111

Background

MAP4K4 is the serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. MAP4K4 appears to act upstream of the JUN N-terminal pathway.The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HGK/MAP4K4 Antibody (NBP1-58853)(1)

We have publications tested in 1 confirmed species: Rabbit.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP4K4