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Granzyme K Antibody (2471A) [Unconjugated]

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Western blot shows lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Granzyme K at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme K Monoclonal Antibody ...read more
Granzyme K was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) at 5 µg/mL for 1 hour at room temperature followed ...read more
Detection of Granzyme K in expanded Human NK cells by Flow Cytometry. NK cells were expanded from Human peripheral blood mononuclear cells (PBMCs) for 14 days using ExCellerate™ Human NK Cell Expansion Media ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
2471A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC10216U-100UG
Alexa Fluor 405
IC10216V-100UG
Alexa Fluor 488
IC10216G-100UG
Alexa Fluor 532
FAB10216X
Alexa Fluor 594
IC10216T-100
Alexa Fluor 647
IC10216R-100UG
Alexa Fluor 700
IC10216N-100UG
Alexa Fluor 750
IC10216S-100UG
Allophycocyanin
FAB10216A
Allophycocyanin/Cy7
FAB10216APCCY7
Biotin
FAB10216B
CoraFluor 1
FAB10216CL1
DyLight 350
FAB10216D
DyLight 405
FAB10216E
DyLight 488
FAB10216K
DyLight 550
FAB10216L
DyLight 594
FAB10216M
DyLight 650
FAB10216W
DyLight 680
FAB10216Y
DyLight 755
FAB10216Z
FITC
FAB10216F
HRP
FAB10216H
Janelia Fluor 525
FAB10216JF525
Janelia Fluor 549
FAB10216I
Janelia Fluor 585
FAB10216JF585
Janelia Fluor 635
FAB10216JF635
Janelia Fluor 646
FAB10216J
Janelia Fluor 669
FAB10216JF669
PE
FAB10216P
PE/Atto594
FAB10216PEATT594
PE/Cy5.5
FAB10216PECY55
PE/Cy7
FAB10216PECY7
PerCP
FAB10216C
mFluor Violet 450 SE
FAB10216MFV450
mFluor Violet 500 SE
FAB10216MFV500
mFluor Violet 610 SE
FAB10216MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC10216N-100UG
Carrier Free
IC10216S-100UG
Carrier Free
IC10216U-100UG
Carrier Free
IC10216R-100UG
Carrier Free
IC10216G-100UG
Carrier Free
IC10216V-100UG
Carrier Free
IC10216T-100
Carrier Free
MAB10216-SP
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Granzyme K Antibody (2471A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide containing human Granzyme K
Accession # P49863
Specificity
Detects human Granzyme K in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme K Antibody (2471A) [Unconjugated]

  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.4
  • Fragmentin-3
  • Granzyme 3
  • granzyme K (granzyme 3; tryptase II)
  • granzyme K (serine protease, granzyme 3; tryptase II)
  • Granzyme K
  • Granzyme-3
  • Granzyme-K
  • GZMK
  • NK-Tryp-2
  • NK-Tryptase-2
  • PRSS
  • TRYP2
  • TRYP2granzyme-3
  • Tryptase II

Background

Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells.  They are serine proteases that induce apoptosis in the target cell.  Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state.  Granzymes have also been found to protect the body against the formation of different kinds of lymphomas.
  1. Bots, M. and JP Medema (2006). J.Cell Sci. 119:5011.
  2. Walch, M. et al. (2014). Cell. 157:1309.
  3. Cullen, SP. et al. (2010). Cell Death Differ. 17:616.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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