![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Immunocytochemical labeling of GM130/GOLGA2 in C2C12 cells. The cells were double-labeled with GM130/GOLGA2 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.](http://images.novusbio.com/fullsize/nbp3-23353_mouse-gm130-golga2-mab-m342-19920231240370.jpg "Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Immunocytochemical labeling of GM130/GOLGA2 in C2C12 cells. The cells were double-labeled with GM130/GOLGA2 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | M342 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130/GOLGA2. This sequence has high homology to similar regions in rat and mouse GM130/GOLGA2. [UniProt# Q08379] |
| Specificity | This antibody detects a 130 kDa protein corresponding to the apparent molecular mass of GM130/GOLGA2 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GOLGA2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 130 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | GOLGA2 |
![Western Blot: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Western blot analysis of GM130/GOLGA2 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130/GOLGA2 (C-terminal region) at 1:500.](http://images.novusbio.com/fullsize/nbp3-23353_mouse-gm130-golga2-mab-m342-1192023166539.jpg "Western Blot: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Western blot analysis of GM130/GOLGA2 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130/GOLGA2 (C-terminal region) at 1:500.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")