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GM130/GOLGA2 Antibody (JE42-53)

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Western Blot: GM130/GOLGA2 Antibody (JE42-53) [NBP3-32394] - Western blot analysis of GM130/GOLGA2 on MCF-7 cell lysates with Rabbit anti-GM130/GOLGA2 antibody (NBP3-32394) at 1/500 dilution. Lysates/proteins at 10 ...read more
Immunohistochemistry: GM130/GOLGA2 Antibody (JE42-53) [NBP3-32394] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GM130/GOLGA2 antibody (NBP3-32394) at 1/1,000 dilution. The ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
JE42-53
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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GM130/GOLGA2 Antibody (JE42-53) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein within human GM130/GOLGA2 aa 1-100 (N terminal). (Uniprot: Q08379)
Localization
Cytoplasm, Cytoskeleton, Membrane, Microtubule.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
GOLGA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:500
Theoretical MW
113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GM130/GOLGA2 Antibody (JE42-53)

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Background

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOLGA2