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Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric)

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ELISA: Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68014] - Samples were spiked with high concentrations of Human Glutathione Peroxidase 1/GPX1 and diluted with Reference Standard & Sample Diluent ...read more
ELISA: Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68014] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

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Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human GPX1 in samples. No significant cross-reactivity or interference between Human GPX1 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.69%
Intra-Assay
CV% < 4.92%
Spike Recovery
86-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GPX1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric)

  • Cellular glutathione peroxidase
  • CGPx
  • EC 1.11.1
  • EC 1.11.1.9
  • Glutathione Peroxidase 1
  • GPX1
  • GPx-1
  • GSHPX1
  • GSHPx-1
  • MGC14399
  • MGC88245

Background

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GPX1