I want to measure SOD and Catalase on different erythroid subpopulation by using flow cytometry.I want to know whether these SOD and Catalase molecules are directly conjugated with fluorescence molecules or I have to do indirect staining.I have to use Ter 119 and CD71 molecule along with catalase or SO antibody.Can you please provide me detail protocol for using these antibodis or send me some references, if anyone used these antibodies by utilizing multicolour flow cytometry.

For our testing of NB100-1992, we used conjugated secondary antibodies. We have not yet tried to directly conjugate this primary, although this is certainly possible and would be expected to give the same results. We do not routinely test our antibodies in FACS, and in most cases, this application is validated by a trusted collaborator. For NB100-1992, viability for FACS was reported by a customer, and we do not have a detailed protocol for this application and product.

What is your recommended working dilution for IHC?

In regards to the IHC dilution, we would recommend starting at about 1:10-1:2000. Obviously this will depend on your samples and how highly expressed the protein is in them. You may need to optimize, but this is a good starting point.

SOD2/Mn-SOD Antibody (NB100-1992): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gp, Gt, Ha, Pm, Rb, Sh, Sq, XpSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC, IP
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Immunogen	Recombinant Rat Mn SOD Protein
Localization	Mitochondrial matrix
Specificity	Detects 25 kDa protein, corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS-PAGE immunoblots.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	SOD2
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7.4), 50% Glycerol
Preservative	0.09% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Publications using NB100-1992	Applications	Species
Han B, Zhao H, Gong X et al. Upregulation of PGC-1 alpha Attenuates Oxygen-Glucose Deprivation-Induced Hippocampal Neuronal Injury Neural plasticity 2022-06-09 [PMID: 35719138] (ICC/IF, Mouse)	ICC/IF	Mouse
Messner H An in vivo, ex vivo, and in vitro exploration of the use of chronic hypoxia/physioxia and ROS/RNS-mediated alteration of physiological function in mitochondrial disease Thesis 2020-01-01 (WB, Human)	WB	Human
Chen H, Zhou J, Chen H et al. Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4 Clinical and translational medicine 2022-04-01 [PMID: 35390228] (WB, Mouse)	WB	Mouse
Kurobe N, Kato K. Biomed. Rsch. 12:97-103. 1991-01-01
Qi XY, Yuan JD, Liu ZY et al. Sirtuin 3-mediated deacetylation of superoxide dismutase 2 ameliorates sodium fluoride-induced mitochondrial dysfunction in porcine oocytes The Science of the total environment 2024-01-15 [PMID: 37944611]
Gu X, Zhao L, Ye J et al. 1,25(OH)2D3 ameliorates doxorubicin?induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress Experimental and Therapeutic Medicine 2023-07-11 [PMID: 37559932] (WB, Mouse) Details: 1:1000 dilution	WB	Mouse
Braun JL, Messner HN, Cleverdon REG et al. Heterozygous SOD2 deletion selectively impairs SERCA function in the soleus of female mice Physiological Reports 2022-05-17 [PMID: 35581738] (WB, IP)	WB, IP
Peng L, Jiang J, Chen HN Et al. Redox-sensitive cyclophilin A elicits chemoresistance through realigning cellular oxidative status in colorectal cancer Cell reports 2021-11-30 [PMID: 34852234] (WB, Human)	WB	Human
Suresh V, Mohanty V, Avula K et al. Quantitative proteomics of hamster lung tissues infected with SARS-CoV-2 reveal host factors having implication in the disease pathogenesis and severity FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-07-01 [PMID: 34105201] (IF/IHC, Hamster)	IF/IHC	Hamster
Dai Z, Li D, Du X et al. Drosophila Caliban preserves intestinal homeostasis and lifespan through regulating mitochondrial dynamics and redox state in enterocytes PLOS Genetics 2020-10-15 [PMID: 33057338]
Yu C, Chen S, Wang X et al. Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring Microvasc. Res. 2020-09-18 [PMID: 32956647] (WB, Rat)	WB	Rat
Zhang Q, Li J, Li Y et al. Bmi deficiency causes oxidative stress and intervertebral disc degeneration which can be alleviated by antioxidant treatment J. Cell. Mol. Med. 2020-06-24 [PMID: 32583517] (WB, Mouse)	WB	Mouse
Zhang H, Ma Y, Wang M et al. Dietary supplementation of l-arginine and N-carbamylglutamate enhances duodenal barrier and mitochondrial functions and suppresses duodenal inflammation and mitophagy in suckling lambs suffering from intrauterine-growth-restriction Food Funct. 2020-04-27 [PMID: 32374309]
Aral C, Demirkesen S, Bircan R, Yasar Sirin D Melatonin reverses the oxidative stress and mitochondrial dysfunction caused by LETM1 silencing Cell Biol. Int. 2019-11-27 [PMID: 31777134] (WB, Mouse)	WB	Mouse
Sun H, Qiao W, Cui M et al. The Polycomb Protein Bmi1 Plays a Crucial Role in the Prevention of 1,25(OH)2 D Deficiency-induced Bone Loss J Bone Miner Res. 2019-11-14 [PMID: 31725940] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Chen L, Yang R et al. 1,25-Dihydroxyvitamin D exerts an antiaging role by activation of Nrf2-antioxidant signaling and inactivation of p16/p53-senescence signaling. Aging Cell 2019-01-06 [PMID: 30907059] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang H, Jin Y, Wang M et Al. N-Carbamylglutamate and l-arginine supplementation improve hepatic antioxidant status in intrauterine growth-retarded suckling lambs RSC Adv. 2020-03-18 [PMID: 30869672]
Xie MY, Yang Y, Liu P et al. 5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina. Int J Ophthalmol 2019-01-18 [PMID: 30662833] (WB, Rat)	WB	Rat
Du Y, Gu X, Meng H et al. Muscone improves cardiac function in mice after myocardial infarction by alleviating cardiac macrophage-mediated chronic inflammation through inhibition of NF-kB and NLRP3 inflammasome. Am J Transl Res 2018-12-15 [PMID: 30662666] (WB, Mouse)	WB	Mouse
Geng Q, Gao H, Yang R et al. Pyrroloquinoline Quinone Prevents Estrogen Deficiency-Induced Osteoporosis by Inhibiting Oxidative Stress and Osteocyte Senescence Int. J. Biol. Sci. 2019-01-01 [PMID: 30662347] (WB, Mouse)	WB	Mouse
Guo YX, Zhang GM, Yao XL et al. Effects of nitric oxide on steroidogenesis and apoptosis in goat luteinized granulosa cells Theriogenology 2018-12-03 [PMID: 30530158] (WB, Goat)	WB	Goat
Chowdhury A, Aich A, Jain G et al. Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1a Expression in Hypoxia. Cell Rep. 2018-10-16 [PMID: 30332638] (Mouse)		Mouse
Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. 2018-09-21 [PMID: 30298003] (WB, Chicken)	WB	Chicken
Guo Y, Nie H, Zhang G, Sun L. Effects of nutrient restriction and arginine treatment on oxidative stress in the ovarian tissue of ewes during the luteal phase. Theriogenology. 2018-02-01 [PMID: 29501008] (Sheep)		Sheep
Chen YH, Lin WW, Liu CS, Su SL. H2O2 induces caveolin-1 degradation and impaired mitochondrial function in E11 podocytes Mol Med Rep 2017-09-18 [PMID: 28944844] (WB, Human)	WB	Human
Yao X, Zhang G, Guo Y et al. Vitamin D receptor expression and potential role of vitamin D on cell proliferation and steroidogenesis in goat ovarian granulosa cells Theriogenology 2017-08-03 [PMID: 28797922] (WB, Goat)	WB	Goat
Chen YH, Lin WW, Liu CS et al. Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia. PLoS ONE. 2016-04-29 [PMID: 27124120] (IHC-P, WB, Rabbit)	IHC-P, WB	Rabbit
Zhang Y, Chen Y, Gucek M, Xu H. The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication. EMBO J. 2016-04-06 [PMID: 27053724] (WB, Drosophila)	WB	Drosophila
Chung C, Puthanveetil P, Ory DS, Lieberman AP Genetic and pharmacological evidence implicates cathepsins in Niemann-Pick C cerebellar degeneration Hum Mol Genet. 2016-03-31 [PMID: 26908626] (IHC-P, Mouse)	IHC-P	Mouse
Freitas I, Boncompagni E, Tarantola E et al. In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet. Oxid Med Cell Longev 2016-01-06 [PMID: 26881047] (WB)	WB
Chen YH, Lin WW, Liu CS et al. Caveolin-1 provides palliation for adverse hepatic reactions in hypercholesterolemic rabbits. PLoS ONE 2014-01-29 [PMID: 24475013] (WB, IHC-P, Rabbit)	WB, IHC-P	Rabbit
Chan JK, Charrier JG, Kodani SD et al. Combustion-derived flame generated ultrafine soot generates reactive oxygen species and activates Nrf2 antioxidants differently in neonatal and adult rat lungs. Part Fibre Toxicol 2013-08-01 [PMID: 23902943] (WB, Rat)	WB	Rat
Hagopian K, Tomilov AA, Tomilova N et al. Shc proteins influence the activities of enzymes involved in fatty acid oxidation and ketogenesis Metabolism 2012-06-07 [PMID: 22683097] (WB, Mouse)	WB	Mouse
Honda F, Kano H, Kanegane H, Nonoyama S, Kim ES, Lee SK, Takagi M, Mizutani S, Morio T. The kinase Btk negatively regulates the production of reactive oxygen species and stimulation-induced apoptosis in human neutrophils. Nat Immunol doi: 10 1038/ni 2234. 2012-02-26 [PMID: 22366891]
Dumont M, Wille E, Stack C et al. Reduction of oxidative stress, amyloid deposition, memory deficit by manganese superoxide dismutase overexpression in a transgenic mouse model of Alzheimer's disease. FASEB J :fj.09-132928. 2009-01-01 [PMID: 19346295]
Furukawa Y, O’Halloran T. Antioxidants & Redo Signaling. 8( 5): 6. 2006-01-01 [PMID: 16771675]
Gao B et al. Am J Physiol Lung. Cell Mol Physiol 284: L917-L925. 2003-01-01 [PMID: 12736188]
Xiao-Hong Liu et al. Brain Res. 625: 29-37. 1993-01-01 [PMID: 7694776]
Bannister JV, Bannister WH, Rotilio G. Aspects of the structure, function, and applications of superoxide dismutase. CRC Crit Rev Biochem;22(2):111-80. 1987-01-01 [PMID: 3315461]
Hassan HM. Free Radical Biol. Med. 5: 377-385. 1988-01-01 [PMID: 2855738]
Wispe JR, Clark JC, Burhans MS et al. Synthesis and processing of the precursor for human mangano-superoxide dismutase. BBA. 994: 30-36. 1989-01-01 [PMID: 2462451]
Adachi T, Ohta H, Yamada H et al. Quantitative analysis of extracellular-superoxide dismutase in serum and urine by ELISA with monoclonal antibody. Clin. Chim. Acta. 212: 89-102. 1992-01-01 [PMID: 1477980]
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SOD2/Mn-SOD Antibody

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Gene Symbol	SOD2
Entrez	6648(Human) 20656(Mouse) 24787(Rat)
Uniprot	P07895(Rat)