![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat cerebellum. GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-2120249403884.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat cerebellum. GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | Hu, Mu, Rt, Ca, Fe, Po, Bv, Ch, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1095 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GAD1/GAD67. The exact sequence is proprietary. |
| Predicted Species | Porcine (99%), Chimpanzee (100%), Bovine (98%), Chicken (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GAD1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GAD1/GAD67 Antibody (NBP3-25474)Find related products by research area.
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| Gene Symbol | GAD1 |
![Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-212024940832.jpg "Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-212024943289.jpg "Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-2120249403812.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-2120249374989.jpg "Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-2120249342780.jpg "Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:500. Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-2120249403832.jpg "Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:500. Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500.")
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25474_rabbit-gad1-gad67-mab-hl1095-azide-and-bsa-free-2120249413398.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")