![Western Blot: Fluorescein Antibody [NB600-493] - Lane 1: BSA conjugated Fluoresccein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 67 kDa, 67 kDa for Fluorescein. Other band(s): None.](http://images.novusbio.com/fullsize/Fluorescein-Antibody-Western-Blot-NB600-493-img0004.jpg "Western Blot: Fluorescein Antibody [NB600-493] - Lane 1: BSA conjugated Fluoresccein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 67 kDa, 67 kDa for Fluorescein. Other band(s): None.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB600-969 | |||
| Biotin | NB100-2082 | |||
| HRP | NB120-6656 | |||
| Rhodamine | NBP1-97384 | |||
| Texas Red | NB110-3648-100ug | |||
| Description | Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. Store vial at 4C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | This product has been tested by ELISA and western blot and is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Use in IHC-FrFl reported in scientific literature (PMID:35411060) Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 30228102). |
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Fluorescein Antibody [NB600-493] - Analysis using the Biotin conjugate of NB600-493. Detection of Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1000 for 60 min at RT.](http://images.novusbio.com/fullsize/Fluorescein-Antibody-Western-Blot-NB600-493-img0003.jpg "Western Blot: Fluorescein Antibody [NB600-493] - Analysis using the Biotin conjugate of NB600-493. Detection of Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1000 for 60 min at RT.")
