![Dot Blot: Fluorescein Antibody [HRP] [NB120-6656]](http://images.novusbio.com/fullsize/Fluorescein-Antibody-[HRP]-Dot-Blot-NB120-6656-img0002.jpg "Dot Blot: Fluorescein Antibody [HRP] [NB120-6656]")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, DB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | HRP |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB600-969 | |||
| Biotin | NB100-2082 | |||
| Rhodamine | NBP1-97384 | |||
| Texas Red | NB110-3648-100ug | |||
| Unconjugated | NB600-493 | |||
| Description | For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room Fluorescein Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Fluorescein conjugated IgG. |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by ELISA, dot blot, and western blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and in situ hybridization, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
| Readout System |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Dot Blot Results of Fluorescein Antibody [HRP]. Blot row top (T): FITC-BSA conjugated. Blot row bottom (B): BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Fluorescein Antibody HRP at 1ug/mL in 1hr RT. Imaged with BioRad ChemiDoc, DyLight 549 filter.](http://images.novusbio.com/fullsize/nb120-6656_goat-polyclonal-fluorescein-antibody-hrp-235202317492111.jpg "Dot Blot Results of Fluorescein Antibody [HRP]. Blot row top (T): FITC-BSA conjugated. Blot row bottom (B): BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Fluorescein Antibody HRP at 1ug/mL in 1hr RT. Imaged with BioRad ChemiDoc, DyLight 549 filter.")
