![Western Blot: Fluorescein Antibody [Biotin] [NB100-2082] - Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for BSA-FITC. Other band(s): None.](http://images.novusbio.com/fullsize/Fluorescein-Antibody-[Biotin]-Western-Blot-NB100-2082-img0003.jpg "Western Blot: Fluorescein Antibody [Biotin] [NB100-2082] - Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for BSA-FITC. Other band(s): None.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Biotin |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB600-969 | |||
| HRP | NB120-6656 | |||
| Rhodamine | NBP1-97384 | |||
| Texas Red | NB110-3648-100ug | |||
| Unconjugated | NB600-493 | |||
| Description | For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum and Fluorescein conjugated IgG. |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by ELISA, dot blot and western blot and is suitable for immunoblotting, ELISA, in situ hybridization, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. |
| Readout System |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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