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Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, ELISA(Comp)
Clone
H22
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-81098AF350
Alexa Fluor 405
NBP2-81098AF405
Alexa Fluor 488
NBP2-81098AF488
Alexa Fluor 532
NBP2-81098AF532
Alexa Fluor 594
NBP2-81098AF594
Alexa Fluor 647
NBP2-81098AF647
Alexa Fluor 700
NBP2-81098AF700
Alexa Fluor 750
NBP2-81098AF750
Allophycocyanin
NBP2-81098APC
Biotin
NBP2-81098B
CoraFluor 1
NBP2-81098CL1
DyLight 350
NBP2-81098UV
DyLight 405
NBP2-81098V
DyLight 488
NBP2-81098G
DyLight 550
NBP2-81098R
DyLight 594
NBP2-81098DL594
DyLight 650
NBP2-81098C
DyLight 680
NBP2-81098FR
DyLight 755
NBP2-81098IR
FITC
NBP2-81098F
HRP
NBP2-81098H
Janelia Fluor 549
NBP2-81098JF549
Janelia Fluor 585
NBP2-81098JF585
Janelia Fluor 635
NBP2-81098JF635
Janelia Fluor 646
NBP2-81098JF646
Janelia Fluor 669
NBP2-81098JF669
PE
NBP2-81098PE
PerCP
NBP2-81098PCP
Unconjugated
NBP2-81098-0.2mg
mFluor Violet 450 SE
NBP2-81098MFV450
mFluor Violet 500 SE
NBP2-81098MFV500
mFluor Violet 610 SE
NBP2-81098MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-81098-0.2mg
Azide and BSA Free
NBP2-81098-0.2mg
Azide and BSA Free
NBP2-81098-0.025mg
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Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.
Specificity
This immunotoxin is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
FCGR1A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Competitive ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]

  • CD64 antigen
  • CD64
  • CD64a
  • Fc fragment of IgG, high affinity Ia, receptor (CD64)
  • Fc gamma RI
  • FCG1
  • Fc-gamma receptor I B2
  • Fc-gamma RI
  • Fc-gamma RIA
  • FcgammaRIa
  • FCGR1
  • FcgRI
  • FcgRIA
  • FCRI
  • FcRIA
  • FLJ18345
  • high affinity Ia, receptor for (CD64)
  • high affinity immunoglobulin gamma Fc receptor I
  • IgG Fc receptor I

Background

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-81098JF525
Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]
Species: Hu
Applications: Flow, ICC/IF, ELISA(Comp)

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FCGR1A