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FABP4/A-FABP Antibody [Unconjugated]

 (18 Publications)
 (1 Review)
 
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Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ...read more
FABP4/A-FABP was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) at 3 µg/mL ...read more
Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 19 kDa (as indicated) using 10 µg/mL of Goat ...read more
Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (MAB3150) coated on a Clear Polystyrene Microplate (DY990). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3150-SP
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FABP4/A-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FABP4/A‑FABP
Cys2-Ala132
Accession # P15090
Specificity
Detects human FABP4/A‑FABP in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse (rm) FABP4 is observed, approximately 30% cross‑reactivity with recombinant human (rh) FABP3 is observed, and less than 5% cross‑reactivity with rhFABP1, -2, -5, -6, -7, -8, -9, and rmFABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3150 in the following applications:
Publications
Read Publications using
AF3150 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP4/A-FABP Antibody [Unconjugated]

  • Adipocyte lipid-binding protein
  • Adipocyte-type fatty acid-binding protein
  • AFABP
  • A-FABP
  • A-FABPAFABP
  • ALBP
  • aP2
  • FABP4
  • fatty acid binding protein 4, adipocyte
  • Fatty acid-binding protein 4
  • fatty acid-binding protein, adipocyte

Background

FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP4/A-FABP Antibody (AF3150)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


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ICC
(7)
IHC
(2)
Western Blot
(3)
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Human
(11)
Mouse
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF3150 Applications Species
Gomez-Sequeda, N;Mendivil-Perez, M;Jimenez-Del-Rio, M;Lopera, F;Velez-Pardo, C; Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology Scientific reports 2023-08-08 [PMID: 37553376] (ICC, Human) ICC Human
K Jabbari, Q Cheng, G Winkelmaie, S Furuta, B Parvin CD36+ Fibroblasts Secrete Protein Ligands That Growth-Suppress Triple-Negative Breast Cancer Cells While Elevating Adipogenic Markers for a Model of Cancer-Associated Fibroblast International Journal of Molecular Sciences, 2022-10-22;23(21):. 2022-10-22 [PMID: 36361532] (ICC, Human) ICC Human
Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs iScience 7/15/2022 [PMID: 35754733]
C Bosc, N Broin, M Fanjul, E Saland, T Farge, C Courdy, A Batut, R Masoud, C Larrue, S Skuli, N Espagnolle, JC Pagès, A Carrier, F Bost, J Bertrand-M, J Tamburini, C Récher, S Bertoli, V Mansat-De, S Manenti, JE Sarry, C Joffre Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites Nat Commun, 2020-08-13;11(1):4056. 2020-08-13 [PMID: 32792483] (Western Blot, Human) Western Blot Human
C Liang, Z Liu, M Song, W Li, Z Wu, Z Wang, Q Wang, S Wang, K Yan, L Sun, T Hishida, Y Cai, JCI Belmonte, P Guillen, P Chan, Q Zhou, W Zhang, J Qu, GH Liu Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration Cell Res., 2020-07-31;0(0):. 2020-07-31 [PMID: 32737416] (ICC, Human) ICC Human
Shijia Bi, Zunpeng Liu, Zeming Wu, Zehua Wang, Xiaoqian Liu, Si Wang et al. SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer Protein & Cell 7/1/2020 [PMID: 32504224]
Josefa Girona, Roser Rosales, Paula Saavedra, Lluís Masana, Joan-Carles Vallvé Palmitate decreases migration and proliferation and increases oxidative stress and inflammation in smooth muscle cells: role of the Nrf2 signaling pathway American Journal of Physiology-Cell Physiology 6/1/2019 [PMID: 30865473]
W Hu, C Jiang, D Guan, P Dierickx, R Zhang, A Moscati, GN Nadkarni, DJ Steger, RJF Loos, C Hu, W Jia, RE Soccio, MA Lazar Patient Adipose Stem Cell-Derived Adipocytes Reveal Genetic Variation that Predicts Antidiabetic Drug Response Cell Stem Cell, 2019-01-10;24(2):299-308.e6. 2019-01-10 [PMID: 30639037] (Western Blot, Human) Western Blot Human
F Yan, N Shen, J X Pang, N Zhao, Y W Zhang, A M Bode et al. A vicious loop of fatty acid-binding protein 4 and DNA methyltransferase 1 promotes acute myeloid leukemia and acts as a therapeutic target Leukemia 4/1/2018 [PMID: 28993705]
EWY Tung, S Ahmed, V Peshdary, E Atlas Firemaster� 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (Ppar?) on the adipocyte protein 2 (aP2) promoter PLoS ONE, 2017-04-24;12(4):e0175855. 2017-04-24 [PMID: 28437481] (Western Blot, Mouse) Western Blot Mouse
Show All 18 Publications.

Review for FABP4/A-FABP Antibody (AF3150) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3150:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IF Human 01/07/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23423575
SpeciesHuman

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 Verified Customer
01/07/2015
Application: IF
Species: Human
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Bioinformatics

Gene Symbol FABP4
Uniprot