![Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0008.jpg "Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERF. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Rat (99%), Bovine (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ERF |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 59 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ERF Antibody (NBP2-16366)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: ERF Antibody [NBP2-16366] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERF stained by ERF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16366-img0004.jpg "Immunocytochemistry/Immunofluorescence: ERF Antibody [NBP2-16366] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERF stained by ERF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - Mouse testis. ERF stained by ERF antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-Paraffin-NBP2-16366-img0003.jpg "Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - Mouse testis. ERF stained by ERF antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: ERF Antibody [NBP2-16366] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0006.jpg "Western Blot: ERF Antibody [NBP2-16366] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - U373 xenograft, using ERF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-Paraffin-NBP2-16366-img0002.jpg "Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - U373 xenograft, using ERF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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