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ESD Antibody

Images

 
Western Blot: ESD Antibody [NBP2-16374] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ESD Antibody [NBP2-16374] - Confocal immunofluorescence analysis of methanol-fixed A431, using ESD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with ...read more
Immunohistochemistry-Paraffin: ESD Antibody [NBP2-16374] - ESD antibody detects ESD protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ESD antibody diluted at 1:500.
Western Blot: ESD Antibody [NBP2-16374] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry: ESD Antibody [NBP2-16374] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ESD antibody (NBP2-16374) at 1:300 dilution.
Western Blot: ESD Antibody [NBP2-16374] - Sample (30 ug of whole cell lysate) A: whole zebrafish 12% SDS PAGE NBP2-16374 diluted at 1:1000

Product Details

Summary
Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ESD Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ESD. The exact sequence is proprietary.
Localization
Cytoplasm; Cytoplasmic vesicle
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ESD
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (82%), Zebrafish (83%), Bovine (89%), Xenopus tropicalis (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for ESD Antibody

  • EC 3.1.2.12
  • esterase 10
  • esterase Desterase D/formylglutathione hydrolase
  • FGH
  • FLJ11763
  • S-formylglutathione hydrolase

Background

This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ESD
Uniprot