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![Western Blot: ESD Antibody [NBP2-16374] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ESD-Antibody-Western-Blot-NBP2-16374-img0002.jpg "Western Blot: ESD Antibody [NBP2-16374] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: ESD Antibody [NBP2-16374] - Confocal immunofluorescence analysis of methanol-fixed A431, using ESD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.](http://images.novusbio.com/fullsize/ESD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16374-img0003.jpg)
![Immunohistochemistry-Paraffin: ESD Antibody [NBP2-16374] - ESD antibody detects ESD protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ESD antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ESD-Antibody-Immunohistochemistry-Paraffin-NBP2-16374-img0004.jpg)
![Western Blot: ESD Antibody [NBP2-16374] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ESD-Antibody-Western-Blot-NBP2-16374-img0001.jpg)
![Immunohistochemistry: ESD Antibody [NBP2-16374] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ESD antibody (NBP2-16374) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16374_rabbit-polyclonal-esd-antibody-91020242021934.jpg)
![Western Blot: ESD Antibody [NBP2-16374] - Sample (30 ug of whole cell lysate) A: whole zebrafish 12% SDS PAGE NBP2-16374 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16374_rabbit-polyclonal-esd-antibody-91020242041280.jpg)
![Western Blot: ESD Antibody [NBP1-89479] - Analysis in human cell line HL-60.](http://images.novusbio.com/fullsize/ESD-Antibody-Western-Blot-NBP1-89479-img0008.jpg "Western Blot: ESD Antibody [NBP1-89479] - Analysis in human cell line HL-60.")
![Immunocytochemistry/Immunofluorescence: ESD Antibody [NBP1-89479] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/ESD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89479-img0007.jpg)
![Immunohistochemistry-Paraffin: ESD Antibody [NBP1-89479] - Staining of human stomach shows strong dot like cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ESD-Antibody-Immunohistochemistry-Paraffin-NBP1-89479-img0002.jpg)
![Western Blot: ESD Antibody [NBP1-57596] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:1562500 Positive Control: Human kidney](http://images.novusbio.com/fullsize/ESD-Antibody-Western-Blot-NBP1-57596-img0004.jpg "Western Blot: ESD Antibody [NBP1-57596] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:1562500 Positive Control: Human kidney")
![Immunohistochemistry: ESD Antibody [NBP1-57596] - Formalin Fixed Paraffin Embedded Tissue: Human Heart Tissue Observed Staining: Cytoplasm in endothelial and smooth muscle cells in arterioles Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/ESD-Antibody-Immunohistochemistry-NBP1-57596-img0003.jpg)
![Western Blot: ESD Antibody (1E1) [H00002098-M01] - ESD monoclonal antibody (M01), clone 1E1. Analysis of ESD expression in Jurkat.](http://images.novusbio.com/fullsize/ESD-Antibody-1E1-Western-Blot-H00002098-M01-img0005.jpg "Western Blot: ESD Antibody (1E1) [H00002098-M01] - ESD monoclonal antibody (M01), clone 1E1. Analysis of ESD expression in Jurkat.")
![Sandwich ELISA: ESD Antibody (1E1) [H00002098-M01] - Detection limit for recombinant GST tagged ESD is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ESD-Antibody-1E1-Sandwich-ELISA-H00002098-M01-img0004.jpg)
![Western Blot: ESD Antibody (J0-8-R) [NBP3-32319] - Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (NBP3-32319) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: SW480 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32319) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32319_mouse-esd-mab-j0-8-r-27820241631619.jpg "Western Blot: ESD Antibody (J0-8-R) [NBP3-32319] - Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (NBP3-32319) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: SW480 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32319) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ESD Overexpression Lysate (Adult Normal) [NBL1-10342] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ESD.](http://images.novusbio.com/fullsize/ESD-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10342-img0002.jpg "Western Blot: ESD Overexpression Lysate (Adult Normal) [NBL1-10342] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ESD.")
![SDS-Page: ESD Protein [NBP1-30238] - Esterase D, 33.6 kDa (302aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/ESD-Protein-SDS-Page-NBP1-30238-img0001.jpg "SDS-Page: ESD Protein [NBP1-30238] - Esterase D, 33.6 kDa (302aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")