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EN-RAGE/S100A12 Antibody (161212) [Unconjugated]

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Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 2 µg/mL of Human EN-RAGE/S100A12 Monoclonal Antibody (Catalog # MAB10522) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EN-RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 2 µg/mL of Human EN-RAGE/S100A12 Monoclonal Antibody (Catalog # MAB10522) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
161212
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB10522-SP
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EN-RAGE/S100A12 Antibody (161212) [Unconjugated] Summary

Immunogen
E.coli-derived recombinant human EN-RAGE/S100A12
Met1-Glu92
Accession # NP_005612
Specificity
Detects human EN-RAGE/S100A12 in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
S100A12
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EN-RAGE/S100A12 Antibody (161212) [Unconjugated]

  • CAAF1
  • CAAF1Neutrophil S100 protein
  • CAAFI
  • CAGC
  • CAGCS100 calcium binding protein A12 (calgranulin C)
  • CAGCS100
  • Calcium-binding protein in amniotic fluid 1
  • Calgranulin C
  • calgranulin-C
  • CGRPEN-RAGE
  • ENRAGE
  • EN-RAGE
  • Extracellular newly identified RAGE-binding protein
  • MRP6
  • p6calgranulin C
  • protein S100-A12
  • S100 calcium binding protein A12
  • S100 calcium-binding protein A12 (calgranulin C)
  • S100 calcium-binding protein A12
  • S100A12

Background

EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa secreted molecule that belongs to the S100 family of calcium-binding proteins. EN-RAGE contains two EF-hand domains and forms dimers and hexamers in solution. EN-RAGE is overexpressed at sites of inflammation and interacts with RAGE on endothelial cells, lymphocytes, and monocytes to promote inflammatory responses. Human EN-RAGE shares 66% and 70% amino acid sequence identity with bovine and porcine EN-RAGE, respectively; mouse and rat orthologs have not been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol S100A12
Uniprot