![Immunocytochemistry/Immunofluorescence: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF4E2.](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71468-img0005.jpg "Immunocytochemistry/Immunofluorescence: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF4E2.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI1F11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human EIF4E2 (NP_004837) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EIF4E2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 28.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for EIF4E2 Antibody (NBP2-71468)Find related products by research area.
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| Gene Symbol | EIF4E2 |
![Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Human thyroid tissue using anti-EIF4E2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71468-img0009.jpg "Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Human thyroid tissue using anti-EIF4E2 mouse monoclonal antibody.")
![Flow Cytometry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Analysis of Hela cells, using anti-EIF4E2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71468-img0004.jpg "Flow Cytometry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Analysis of Hela cells, using anti-EIF4E2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Analysis of extracts (10ug) from 1 cell line](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Western-Blot-NBP2-71468-img0001.jpg "Western Blot: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Analysis of extracts (10ug) from 1 cell line")
![Western Blot: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E2.](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Western-Blot-NBP2-71468-img0002.jpg "Western Blot: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E2.")
![Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EIF4E2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71468-img0006.jpg "Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EIF4E2 mouse monoclonal antibody.")
![Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Human lymph node tissue using anti-EIF4E2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71468-img0007.jpg "Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Human lymph node tissue using anti-EIF4E2 mouse monoclonal antibody.")
![Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Human prostate tissue using anti-EIF4E2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF4E2-Antibody-OTI1F11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71468-img0008.jpg "Immunohistochemistry: EIF4E2 Antibody (OTI1F11) - Azide and BSA Free [NBP2-71468] - Staining of paraffin-embedded Human prostate tissue using anti-EIF4E2 mouse monoclonal antibody.")
![Western Blot eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81898-img0005.jpg)
![Immunohistochemistry-Paraffin eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Western Blot TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Data TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![Immunohistochemistry Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Immunohistochemistry_11792.jpg)
![Western Blot Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Western_Blot_9526.jpg)
![Simple Western Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Simple_Western_20094.jpg)
![Immunocytochemistry RSK1 [p Ser380] Antibody (1024A) [Unconjugated]](https://images.novusbio.com/images2/RSK1_MAB79671_Immunocytochemistry_15894.jpg)
![Simple Western RSK1 [p Ser380] Antibody (1024A) [Unconjugated]](https://images.novusbio.com/images2/16242.jpg)
![Western Blot RSK1 [p Ser380] Antibody (1024A) [Unconjugated]](https://images.novusbio.com/images2/RSK1_MAB79671_Western_Blot_14193.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")