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ARIH1 Antibody

Images

 
Western Blot: ARIH1 Antibody [NB100-1162] - (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: ARIH1 Antibody [NB100-1162] - staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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ARIH1 Antibody Summary

Immunogen
Peptide with sequence C-HEGYEKDLWEYIED corresponding to C-Terminus according to NP_005735.2.
Predicted Species
Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARIH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 1 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in U937 lysate (predicted MW of approx. 61 kDa band according to NP_005735).
IHC: Paraffin embedded Human Adrenal Gland.
Publications
Read Publication using NB100-1162.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARIH1 Antibody

  • ARI-1
  • ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1(Drosophila)
  • ariadne, Drosophila, homolog of
  • ARIMOP-6
  • FLJ20329
  • FLJ93118
  • H7-AP2
  • HARI
  • Monocyte protein 6
  • MOP6
  • protein ariadne-1 homolog
  • UbcH7-binding protein
  • UBCH7BPariadne (Drosophila) homolog, ubiquitin-conjugating enzyme E2-binding protein1,HHARIDKFZp686O13120
  • UbcM4-interacting protein
  • Ubiquitin-conjugating enzyme E2-binding protein 1

Background

Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARIH1 Antibody (NB100-1162)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol ARIH1
Entrez
Uniprot