Novus Biologicals products are now on bio-techne.com

Rat EAAT3 ELISA Kit (Colorimetric)

Images

 
ELISA: Rat EAAT3 ELISA Kit (Colorimetric) [NBP2-82471] - Samples were spiked with high concentrations of Rat EAAT3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat EAAT3 ELISA Kit (Colorimetric) [NBP2-82471] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Rat EAAT3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat EAAT3 in samples. No Significant cross-reactivity or interference between Rat EAAT3 and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.77
Intra-Assay
CV% < 5.39
Spike Recovery
85-102%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
SLC1A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat EAAT3 ELISA Kit (Colorimetric)

  • EAAC1
  • EAAC1excitatory amino acid carrier 1
  • EAAT3
  • EAAT3excitatory amino acid transporter 3
  • Excitatory amino-acid carrier 1
  • Neuronal and epithelial glutamate transporter
  • SLC1A1
  • Sodium-dependent glutamate/aspartate transporter 3
  • solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1
  • Solute carrier family 1 member 1

Background

The EAAT3 gene encodes for glutamate transporters (also known as excitatory amino acid transporters, EAATs), that rapidly move glutamate from the postsynaptic cleft through the plasma membrane. The proteins are approximately 542 amino acids long at a mass barely over 57 kDA. This function allows for neurotoxic levels of glutamate in the brain to be avoided. It is common to see underactivity of glutamate receptors in some disorders such as ischemia and traumatic brain injuries and overactivity to be linked to mental illnesses such as schizophrenia. EAAT3 gene is also related to obsessive-compulsive disorder, ganglioglioma, autism spectrum disorders, aminoaciduriam temporal lobe epilepsy, hepatic encephalopathy, neuronitis, anorexia nervosa, and amyotrophic lateral sclerosis. It interacts with KNCN, HCCS, EWSR1, PRKCA, and ARL6IP5 to function in pathways of glutamic acid signaling, transmembrane transport of small molecules, and protein digestion and absorption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1869
Species: Hu, Mu, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-20136
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, In vivo, ISH, WB
NBP3-13360
Species: Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-91803
Species: Hu
Applications: IHC, IHC-P, WB
H00005688-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
NBP3-12158
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-81802
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-88191
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NB110-41404
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP3-13165
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NLS892
Species: Bt, Bv, Ca, Ha, Hu, Mu, Po, Rb, Rt
Applications: ICC, IHC, IHC-P, WB
NBP1-92005
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-75510
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-82471
Species: Rt
Applications: ELISA

Publications for EAAT3 ELISA Kit (NBP2-82471) (0)

There are no publications for EAAT3 ELISA Kit (NBP2-82471).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EAAT3 ELISA Kit (NBP2-82471) (0)

There are no reviews for EAAT3 ELISA Kit (NBP2-82471). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EAAT3 ELISA Kit (NBP2-82471) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EAAT3 Products

Research Areas for EAAT3 ELISA Kit (NBP2-82471)

Find related products by research area.

Blogs on EAAT3

There are no specific blogs for EAAT3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

GFAP Antibody
NB300-141

Contact Information

Product PDFs

Review this Product

Be the first to review our Rat EAAT3 ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC1A1