Cells are supplied in 2 ml quantities (about 1x10 ^ 6 cells/ml) in freezing media (60% DMEM, 20% FBS, 20% DMSO)
Alternate Names for Dicer knockout Mouse embryonic stem cells
DCR1 ES cells
Dicer1 ES cells
Background
The Dicer knockout Mouse embryonic stem cells were derived from Dicer knockout mice. Dicer is essential for the processing of small non-coding RNAs including microRNAs and endogenous small interfering RNAs. These cells are a valuable research tool for scientists interested in studying the small non-coding RNAs in embryonic stem cells and the differentiated cells derived from them.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Product General Protocols
View specific protocols for Dicer knockout Mouse embryonic stem cells (NBP1-96751):
FAQs for Dicer knockout Mouse embryonic stem cells (NBP1-96751). (Showing 1 - 3 of 3 FAQs).
For NBP1-97434, the RIPA buffer contains SDS. Could you let us know the concentration of SDS in RIPA buffer?
The SDS is at 0.1%
We recently ordered NBP1-96751. Which knock out mouse was this cell line generated? We went through the references but wasn't clear enough if the cell line is null/floxed or null/null.
This cell line was generated from Dicer flox/flox mice. I have provided two links below to publications referencing the production of this cell line. This is the most current information we have available from the inventor of the cell line. Please see http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2569885/#B22 or http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1182454/
Are these cells prior to Cre treatment or are they cells that already have been Cre treated and have Dicer knocked out?
Our Dicer knockout mouse embryonic stem cells, NBP1-96751, are supplied treated and already have Dicer knocked out.
![Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - SSEA1 antibody (NB100-1831) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBP1-96751-img0003.jpg "Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - SSEA1 antibody (NB100-1831) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Brightfield Image of Dicer knock out Mouse embryonic stem cell line colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunohistochemistry-NBP1-96751-img0004.jpg "Immunohistochemistry: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Brightfield Image of Dicer knock out Mouse embryonic stem cell line colonies growing directly on a gelatinized tissue culture flask.")
![Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Nanog antibody (NB100-58842) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBP1-96751-img0002.jpg "Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Nanog antibody (NB100-58842) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).")
![Immunocytochemistry LIN-28A Antibody [Unconjugated]](https://images.novusbio.com/images2/LIN-28A_AF3757_Immunocytochemistry_6502.jpg)
![Immunocytochemistry LIN-28A Antibody [Unconjugated]](https://images.novusbio.com/images2/LIN-28A_AF3757_Immunocytochemistry_12362.jpg)
![Western Blot LIN-28A Antibody [Unconjugated]](https://images.novusbio.com/images2/LIN-28A_AF3757_Western_Blot_9318.jpg)
![Simple Western PKR Antibody (HL71/10) [Unconjugated]](https://images.novusbio.com/images2/PKR_MAB1980_Simple_Western_20723.jpg)
![Immunocytochemistry PKR Antibody (HL71/10) [Unconjugated]](https://images.novusbio.com/images2/PKR_MAB1980_Immunocytochemistry_9844.jpg)
![Western Blot PKR Antibody (HL71/10) [Unconjugated]](https://images.novusbio.com/images2/PKR_MAB1980_Western_Blot_20507.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)