DGCR8 knockout Mouse embryonic stem cells

MicroRNAs are abundant, 21-25 nucleotide non-coding RNAs that are important endogenous regulators of gene expression. MicroRNAs work by specifically regulating messenger RNAs (mRNAs), and are predicted to regulate hundreds of genes individually and simultaneously, affecting cellular functions such as differentiation, development, proliferation, and apoptosis. The specific regulation at both the transcription and the translation level opens the possibility to use microRNAs as targets for the development of drugs and for the diagnosis of several human diseases . Novus Biologicals now offers a novel a line of DGCR8 knockout mouse embryonic stem (ES) cells to study the global role of microRNAs. Embryonic stem cells provide a tool for the study of the molecular mechanisms of early mammalian development. The DGCR8 cells have been genetically altered at the locus of the dgcr8 gene such that no functional DGCR8 protein can be produced, resulting in the global, but specific, loss of micro RNAs. The DGCR8 knockout mouse embryonic stem (ES) cells are unique because they allow for the specific study of the global role of microRNAs. Unlike the Dicer cell line, the only other comparable line for studying microRNAs, the DGCR8 cell line appears to be specific for microRNAs, whereas Dicer also affects other classes of small RNAs. Mouse Dicer1 knockout ES cells have been useful for inferring the role for small RNAs in ES cell differentiation. Dicer is required for the maturation of at least two classes of small RNAs: microRNAs and short interfering RNAs (siRNAs). Thus, in studies using Dicer1 knockout ES cells it can be difficult to isolate whether the lack of microRNAs alone are the cause of the observed phenotype. DGCR8 is part of the microprocessor complex, which is composed of the proteins DGCR8 and Drosha. The microprocessor complex handles microRNA processing; unlike Dicer, this complex seems to be specific to microRNAs. Of the two components of the microprocessor complex, Drosha has been reported to have a role in ribosomal RNA processing, possibly in a distinct protein complex, while DGCR8 does not. Therefore, DGCR8 may be the only member of the processing pathway that is specific to microRNAs. These cells are useful for the specific study of microRNA function in both embryonic stem cells as well as derivatives of embryonic stem cells (e.g. differentiated cells from embryonic stem cells).

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We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: In vitro, WB.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBA1-19349	Applications	Species
Freimer JW, Hu TJ, Blelloch R. Decoupling the impact of microRNAs on translational repression versus RNA degradation in embryonic stem cells Elife 2018-07-25 [PMID: 30044225] (In vitro, Mouse)	In vitro	Mouse
Macias S, Cordiner RA, Gautier P et al. DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs. Mol Cell 2015-12-17 [PMID: 26687677] (WB)	WB
Krawczynski K, Najmula J, Bauersachs S, Kaczmarek MM. MicroRNAome of porcine conceptuses and trophoblasts: expression profile of micrornas and their potential to regulate genes crucial for establishment of pregnancy. Biol Reprod. [PMID: 25472924]
Ciaudo C, Jay F, Okamoto I et al. RNAi-Dependent and Independent Control of LINE1 Accumulation and Mobility in Mouse Embryonic Stem Cells. PLoS Genet. 2013-11-01 [PMID: 24244175]
Jiang K, Ren C, Nair VD. MicroRNA-137 represses Klf4 and Tbx3 during differentiation of mouse embryonic stem cells. Stem Cell Res. 2013-09-13 [PMID: 24084696]
Heras SR, Macias S, Plass M et al. The Microprocessor controls the activity of mammalian retrotransposons. Nat Struct Mol Biol. 2013-09-01 [PMID: 23995758]
Stankiewicz TR, Schroeder EK, Kelsey NA et al. C-terminal binding proteins are essential pro-survival factors that undergo caspase-dependent downregulation during neuronal apoptosis. Mol Cell Neurosci 2013-07-13 [PMID: 23859824]
Macias S, Plass M, Stajuda A et al. DGCR8 HITS-CLIP reveals novel functions for the Microprocessor Nat Struct Mol Biol 2012-08-01 [PMID: 22796965]
Havens MA, Reich AA, Duelli DM, Hastings ML. Biogenesis of mammalian microRNAs by a non-canonical processing pathway. Nucleic Acids Research. 2012-01-23 [PMID: 22270084]
Lei Z, van Mil A, van de Vrugt AM. Dgcr8 is Indispensable for Cardiac Lineage Specification in Embryonic Stem Cells Stem CellResearch & Therapy. 2015-01-15 (In vitro) Details: DGCR8 knockout Mouse embryonic stem cells and v6.5 Mouse embryonic stem cells used for experiments involving in vito cardiac differentiation. Dgcr8 KO mouse embryonic stem cells alongwith v6.5 WT controls were also characterized for the loss of Dgcr8 protein (in the Dgcr8 KO-ESCs) with WB, Genotyping, ICC-IF and RNA profiling (Figure 1).	In vitro
Vardapour R, Kehl T, Kneitz S et al. The DGCR8 E518K mutation found in Wilms tumors leads to a partial miRNA processing defect that alters gene expression patterns and biological processes Carcinogenesis 2021-12-17 [PMID: 34919667]
Majumder S, Ren L, Pushpakumar S, Sen U MicroRNA-deficient mouse embryonic stem cells acquire a functional interferon response Elife 2019-04-23 [PMID: 31012846] (WB, Mouse)	WB	Mouse
Wang et al. DGCR8 is essential for microRNA biogenesis silencing of embryonic stem cell self-renewal. Nat Genet; 39(3):380-5. 2007-03-01 [PMID: 17259983] (In vitro)	In vitro
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