Connexin 43/GJA1 Antibody (578618) [Allophycocyanin]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Connexin43 (Cx43; also gap junction alpha -1 protein) is a 41 - 44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.

Showing Publications 1 - 7 of 7.

Publications using FAB7737A	Applications	Species
Monterisi S, Michl J, Hulikova A et al. Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes eLife 9/15/2022 [PMID: 36107487]
AL Hirschberg, I Jakson, C Graells Br, D Salamon, D Ujvari Interaction between insulin and androgen signalling in decidualization, cell migration and trophoblast invasion in vitro Journal of Cellular and Molecular Medicine, 2021-08-31;0(0):. 2021-08-31 [PMID: 34463022] (Flow Cytometry, Human)	Flow Cytometry	Human
Marta Varela-Eirín, Paula Carpintero-Fernández, Agustín Sánchez-Temprano, Adrián Varela-Vázquez, Carlos Luis Paíno, Antonio Casado-Díaz et al. Senolytic activity of small molecular polyphenols from olive restores chondrocyte redifferentiation and promotes a pro-regenerative environment in osteoarthritis Aging (Albany NY) 8/3/2020 [PMID: 32745074]
P Uribe, A Johansson, R Jugdaohsin, JJ Powell, C Magnusson, M Davila, A Westerlund, M Ransjö Soluble silica stimulates osteogenic differentiation and gap junction communication in human dental follicle cells Sci Rep, 2020-06-18;10(1):9923. 2020-06-18 [PMID: 32555274] (Flow Cytometry, Human)	Flow Cytometry	Human
P Ganguly, JJ El-Jawhari, AN Burska, F Ponchel, PV Giannoudis, EA Jones The Analysis of In Vivo Aging in Human Bone Marrow Mesenchymal Stromal Cells Using Colony-Forming Unit-Fibroblast Assay and the CD45lowCD271+ Phenotype Stem Cells Int, 2019-08-01;2019(0):5197983. 2019-08-01 [PMID: 31467563] (Flow Cytometry, Human)	Flow Cytometry	Human
M Varela-Eir, A Varela-Váz, A Guitián-Ca, CL Paíno, V Mato, R Largo, T Aasen, A Tabernero, E Fonseca, M Kandouz, JR Caeiro, A Blanco, MD Mayán Targeting of chondrocyte plasticity via connexin43 modulation attenuates cellular senescence and fosters a pro-regenerative environment in osteoarthritis Cell Death Dis, 2018-12-05;9(12):1166. 2018-12-05 [PMID: 30518918] (Human)		Human
P Szaraz, M Librach, L Maghen, F Iqbal, TA Barretto, S Kenigsberg, A Gauthier-F, CL Librach In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells Stem Cells Int, 2016-03-30;2016(0):7513252. 2016-03-30 [PMID: 27123009] (Flow Cytometry, Human)	Flow Cytometry	Human

Array FAB7737A

• Connexin 43/GJA1 Antibodies
• Connexin 43/GJA1 ELISA Kits
• Connexin 43/GJA1 Lysate
• Connexin 43/GJA1 Proteins