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Complement Component C1qA Antibody (394107) [Unconjugated]

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Complement Component C1qA was detected in immersion fixed paraffin-embedded sections of Human Placenta using Rat Anti-Human Complement Component C1qA Monoclonal Antibody (Catalog # MAB4035) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in cytotrophoblast in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Complement Component C1qA was detected in immersion fixed paraffin-embedded sections of Human Placenta using Rat Anti-Human Complement Component C1qA Monoclonal Antibody (Catalog # MAB4035) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
394107
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4035-SP
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Complement Component C1qA Antibody (394107) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Complement Component C1qA
Lys110-Ala245
Accession # P02745
Specificity
Detects recombinant human Complement Component C1qA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Complement Component C1qB is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
C1QA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C1qA Antibody (394107) [Unconjugated]

  • C1QA complement component 1, q subcomponent, A chain
  • C1QA
  • Complement Component C1qA

Background

C1qA, one of three subunits of complement component C1q, contains a collagen-like domain and a globular C1q domain. Within the globular domain, human C1qA shares 73% and 77% aa sequence identity with mouse and rat C1qA, respectively. Six disulfide-linked heterodimers of C1qA and C1qB associate with three disulfide-linked homodimers of C1qC to form C1q. Deficiency in C1qA is associated with the development of autoimmunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol C1QA
Uniprot