![Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Immunohistochemistry-Paraffin-NB100-77808-img0014.jpg "Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | G10F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Human peripheral blood cells. |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CEACAM8 |
| Purity | IgM purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | It is recommended that CD66b clone G10F5 be titrated for optimal performance for each application. This antibody is CyTOF ready. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | IgM purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Masahiro Hirata |
IHC-P | Human | 07/28/2020 |
Summary
Comments
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reviewed by:
Manuel Flores |
IHC | Human | 06/09/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for CEACAM8/CD66b Antibody (NB100-77808)Find related products by research area.
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| Masahiro Hirata 07/28/2020 |
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| Application: | IHC-P | |
| Species: | Human |
| Manuel Flores 06/09/2017 |
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| Application: | IHC | |
| Species: | Human |
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0012.jpg "Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.")
![Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Immunohistochemistry-NB100-77808-img0013.jpg "Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0001.jpg "Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0007.jpg "Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor(R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0008.jpg "Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor(R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0009.jpg "Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Flow cytometry image submitted by a verified customer review.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0011.jpg "Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verifed customer review.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0010.jpg "Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.")
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