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CD133 Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-16518AF350
Alexa Fluor 405
NB120-16518AF405
Alexa Fluor 488
NB120-16518AF488
Alexa Fluor 532
NB120-16518AF532
Alexa Fluor 594
NB120-16518AF594
Alexa Fluor 647
NB120-16518AF647
Alexa Fluor 700
NB120-16518AF700
Alexa Fluor 750
NB120-16518AF750
Allophycocyanin
NB120-16518APC
Biotin
NB120-16518B
CoraFluor 1
NB120-16518CL1
DyLight 350
NB120-16518UV
DyLight 405
NB120-16518V
DyLight 488
NB120-16518G
DyLight 594
NB120-16518DL594
DyLight 650
NB120-16518C
DyLight 680
NB120-16518FR
DyLight 755
NB120-16518IR
FITC
NB120-16518F
HRP
NB120-16518H
Janelia Fluor 525
NB120-16518JF525
Janelia Fluor 549
NB120-16518JF549
Janelia Fluor 585
NB120-16518JF585
Janelia Fluor 635
NB120-16518JF635
Janelia Fluor 646
NB120-16518JF646
Janelia Fluor 669
NB120-16518JF669
PE
NB120-16518PE
PerCP
NB120-16518PCP
Unconjugated
NB120-16518
mFluor Violet 450 SE
NB120-16518MFV450
mFluor Violet 500 SE
NB120-16518MFV500
mFluor Violet 610 SE
NB120-16518MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB120-16518
BSA Free
NB120-16518
BSA Free
NB120-16518SS
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CD133 Antibody [DyLight 550] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Marker
Stem Cell Marker
Specificity
CD133 - Hematopoietic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PROM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
ELISA: 1/1000. WB: 1/1,000 - 1:2,000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD133 Antibody [DyLight 550]

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD133 Antibody
[DyLight 550]
NB120-16518R
CD133 Antibody [DyLight 550]
Species: Hu, Mu, Rt, Po
Applications: WB, ELISA, Flow, ICC/IF, IHC, ChIP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PROM1