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Cathepsin B Antibody - BSA Free

Images

 
Western Blot: Cathepsin B Antibody [NBP1-19797] - extracts from COLO cells.
Immunocytochemistry/ Immunofluorescence: Cathepsin B Antibody [NBP1-19797] - ICC/IF analysis of HeLa cells using 1:50 dilution of Cathepsin B antibody followed by detection using Dylight 488 labelled secondary antibody ...read more
Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-19797] - Human brain tissue.
Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-19797] - Staining for Cathepsin B in mouse ureter.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Cathepsin B Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of the Cathepsin B human protein (between residues 200-270) [UniProt P07858]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTSB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0 - 2.0 ug/ml
Publications
Read Publications using
NBP1-19797 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: bovine (84%) and chicken (84%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cathepsin B Antibody - BSA Free

  • APP secretase
  • APPS
  • Cathepsin B
  • Cathepsin B1
  • CPSBamyloid precursor protein secretase
  • CTSB
  • cysteine protease
  • EC 3.4.22
  • EC 3.4.22.1

Background

The cathepsin protease family contains several diverse classes of enzymes. Cathepsins B, L, H, K, S and O are part of the cysteine protease class. Cathepsin B is a dimer consisting of a heavy chain and a light chain cross-linked by a disulfide bond and is a lysosomal cysteine protease. Cathepsin B is expressed in luminal epithelial cells and its expression indicates that Cathepsin B is a marker for secretory cell death. Cathepsin B also participates in intracellular degradation and turnover of proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin B Antibody (NBP1-19797)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.


Filter By Application
IF/IHC
(2)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(1)
All Species

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Product General Protocols

View specific protocols for Cathepsin B Antibody (NBP1-19797): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cathepsin B Antibody (NBP1-19797) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cathepsin B Products

Research Areas for Cathepsin B Antibody (NBP1-19797)

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Blogs on Cathepsin B.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

Caspase 11: A novel non-canonical inflammasomes
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands. This regulated process then transmits downstream signals through adaptor molecules ending with the caspase cysteine proteases. Caspas...  Read full blog post.

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Bioinformatics

Gene Symbol CTSB
Entrez
Uniprot