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Carbonic Anhydrase III/CA3 Antibody (CA3/7885)

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Formalin-fixed, paraffin-embedded human skeletal muscle stained with Carbonic Anhydrase III/CA3 Antibody (CA3/7885). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human skeletal muscle stained with Carbonic Anhydrase III/CA3 Antibody (CA3/7885). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Western blot analysis of RH-30 cell lysate using Carbonic Anhydrase III/CA3 Antibody (CA3/7885).
Formalin-fixed, paraffin-embedded human skeletal muscle stained with Carbonic Anhydrase III/CA3 Antibody (CA3/7885). Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CA3/7885
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-27029AF350
Alexa Fluor 405
NBP3-27029AF405
Alexa Fluor 488
NBP3-27029AF488
Alexa Fluor 532
NBP3-27029AF532
Alexa Fluor 594
NBP3-27029AF594
Alexa Fluor 647
NBP3-27029AF647
Alexa Fluor 700
NBP3-27029AF700
Alexa Fluor 750
NBP3-27029AF750
Allophycocyanin
NBP3-27029APC
Biotin
NBP3-27029B
CoraFluor 1
NBP3-27029CL1
DyLight 350
NBP3-27029UV
DyLight 405
NBP3-27029V
DyLight 488
NBP3-27029G
DyLight 550
NBP3-27029R
DyLight 594
NBP3-27029DL594
DyLight 650
NBP3-27029C
DyLight 680
NBP3-27029FR
DyLight 755
NBP3-27029IR
FITC
NBP3-27029F
HRP
NBP3-27029H
Janelia Fluor 525
NBP3-27029JF525
Janelia Fluor 549
NBP3-27029JF549
Janelia Fluor 585
NBP3-27029JF585
Janelia Fluor 635
NBP3-27029JF635
Janelia Fluor 646
NBP3-27029JF646
Janelia Fluor 669
NBP3-27029JF669
PE
NBP3-27029PE
PerCP
NBP3-27029PCP
mFluor Violet 450 SE
NBP3-27029MFV450
mFluor Violet 500 SE
NBP3-27029MFV500
mFluor Violet 610 SE
NBP3-27029MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-27029
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Carbonic Anhydrase III/CA3 Antibody (CA3/7885) Summary

Description
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Immunogen
Recombinant fragment of human Carbonic Anhydrase III/CA3 protein (exact sequence is proprietary)
Localization
Cytoplasm
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CA3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT
  • Western Blot 1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Carbonic Anhydrase III/CA3 Antibody (CA3/7885)

  • CA3
  • CAIII
  • CA-III
  • Car3
  • Carbonate dehydratase III
  • carbonic anhydrase 3
  • Carbonic Anhydrase III
  • carbonic anhydrase III, muscle specific
  • EC 4.2.1.1

Background

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CA3