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Calbindin D/S100G Antibody [Unconjugated]

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Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by ...read more
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat ...read more
Calbindin D was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Calbindin D/S100G Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Calbindin D
Ala2-Asn261
Accession # P05937
Specificity
Detects human Calbindin D in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100G
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 5 ug/mL
Publications
Read Publications using
AF3320 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calbindin D/S100G Antibody [Unconjugated]

  • CABP
  • CABP;CABP1;CABP9K;CALB3;Protein S100-G
  • CABP1
  • CABP28K
  • CABP9K
  • Calbindin 3
  • Calbindin D
  • Calbindin-D
  • Calbindin-D9K
  • S100G

Background

Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calbindin D/S100G Antibody (AF3320)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA Development (Detection), IHC, IHC-P.


Filter By Application
ELISA Development (Detection)
(1)
IHC
(3)
IHC-P
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF3320 Applications Species
Viventi S, Frausin S, Howden SE et al. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia Stem cells translational medicine 3/18/2021 [PMID: 33734599]
Ashley J. Swain, Adriana Galvan, Thomas Wichmann, Yoland Smith Structural plasticity of GABAergic and glutamatergic networks in the motor thalamus of parkinsonian monkeys Journal of Comparative Neurology 2020-06-01 [PMID: 31808567]
M Zhang, Y Wang, J Geng, S Zhou, B Xiao Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice Cell Rep, 2019-02-05;26(6):1419-1431.e4. 2019-02-05 [PMID: 30726728] (IHC, Mouse) IHC Mouse
Allison M Bradbury Cerebrospinal fluid calbindin D concentration as a biomarker of cerebellar disease progression in Niemann-Pick type C1 disease J Pharmacol Exp Ther, 2016-06-15;0(0):. 2016-06-15 [PMID: 27307499] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Bifsha P, Yang J, Fisher R, Drouin J Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet, 2014-12-11;10(12):e1004863. 2014-12-11 [PMID: 25501001] (IHC-P, Mouse) IHC-P Mouse
Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2014-11-24;18(1):145-53. 2014-11-24 [PMID: 25420068] (IHC, Mouse) IHC Mouse
Di Giovannantonio , Luca G, Di Salvio , Michela, Omodei , Daniela, Prakash , Nilima, Wurst , Wolfgang, Pierani , Alessand, Acampora , Dario, Simeone , Antonio Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development, 2013-12-11;141(2):377-88. 2013-12-11 [PMID: 24335253] (IHC, Mouse) IHC Mouse
Di Salvio M, Di Giovannantonio LG, Acampora D Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat. Neurosci., 2010-11-07;13(12):1481-8. 2010-11-07 [PMID: 21057506] (IHC-P, Mouse) IHC-P Mouse

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Secondary Antibodies

 

Isotype Controls

Additional Calbindin D/S100G Products

Blogs on Calbindin D/S100G.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

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Bioinformatics

Gene Symbol S100G
Uniprot