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BNIP3L Antibody

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BNIP3L at approximately 35kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
BNIP3L was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) at 10 µg/mL for 3 hours ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4030-SP
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BNIP3L Antibody Summary

Immunogen
E. coli-derived recombinant human BNIP3L
Ser2-Glu184
Accession # O60238
Specificity
Detects endogenous human, mouse and rat BNIP3L in Western blots. In Western blots, less than 10% cross-reactivity to recombinant human BNIP3L is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BNIP3L
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF4030 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BNIP3L Antibody

  • Adenovirus E1B19K-binding protein B5
  • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A
  • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
  • BCL2/adenovirus E1B 19-kd protein-interacting protein 3a
  • BCL2/adenovirus E1B 19kDa interacting protein 3-like
  • BNIP3a
  • BNIP3H
  • BNIP3L
  • Nip3L
  • NIP3-like protein X
  • Nix
  • NIXBCL2/adenovirus E1B 19kD-interacting protein 3-like

Background

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family. BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain. BNIP3L is a 219 amino acid (aa), 24 kDa (predicted) protein that contains a C-terminal transmembrane domain required for mitochondrial localization, homodimerisation, and regulation of its proapoptotic function. BNIP3L may antagonize the activity of BCL2 family antiapoptic proteins by directly interactacting with proteins such as viral E1B-19K and cellular Bcl-2 and Bcl-xL. BNIP3L shares 56% amino acid sequence identity with BNIP3 and 98% with mouse and rat BNIP3L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for BNIP3L Antibody (AF4030) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF4030:
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Western Blot BNIP3L AF4030
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reviewed by:
Verified Customer
 WB Mouse 10/15/2020
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Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
LotYFN021904A

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Secondary Antibodies

 

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Western Blot BNIP3L AF4030
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 Verified Customer
10/15/2020
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol BNIP3L
Uniprot