![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - IHC-Prediluted [NBP2-44779] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 antibody (1:200), peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44779-img0003.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - IHC-Prediluted [NBP2-44779] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 antibody (1:200), peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH")
| Reactivity | Hu, Po, Pm, Mu(-), Rt(-)Species Glossary |
| Applications | IHC |
| Clone | 8C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein. (Uniprot: P10415 ) |
| Localization | Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. |
| Marker | Apoptosis & Follicular Lymphoma Marker |
| Specificity | This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCL2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | BCL2 |
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