![Bcl-2 Antibody (8C8) [mFluor Violet 610 SE] [NBP2-33313MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.](http://images.novusbio.com/fullsize/nbp2-33313mfv610_mouse-bcl-2-mab-8c8-mfluor-violet-610-se-6720239124830.png "Bcl-2 Antibody (8C8) [mFluor Violet 610 SE] [NBP2-33313MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.")
| Reactivity | Hu, Po, Pm, Mu(-), Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 8C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | mFluor Violet 610 SE |
| Immunogen | A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein. (Uniprot: P10415 ) |
| Localization | Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. |
| Marker | Apoptosis & Follicular Lymphoma Marker |
| Specificity | This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCL2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | BCL2 |
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