![Western Blot: ATG13 Antibody (3E4C8) [NBP2-52419] - Analysis using ATG13 mAb against HEK293 (1) and ATG13 (AA: 339-550)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ATG13-Antibody-3E4C8-Western-Blot-NBP2-52419-img0004.jpg "Western Blot: ATG13 Antibody (3E4C8) [NBP2-52419] - Analysis using ATG13 mAb against HEK293 (1) and ATG13 (AA: 339-550)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, CyTOF-ready |
| Clone | 3E4C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human ATG13 (AA: 339-550) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ATG13 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 56.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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Read full blog post. |
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Animal Models to Study Autophagy By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the... Read full blog post. |
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ATG11 - An important scaffolding protein in autophagosome formation and fusion Autophagy is a cellular mechanism used to regulate cell metabolism and to recycle or degrade damaged organelles and proteins. This is accomplished through the engulfment of cargo in a double-membrane structure called the autophagosome. The autophag... Read full blog post. |
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ULK1 - mammalian homologue of the yeast ATG1 kinase Autophagy is an important cellular process involved in degradation and recycling of cellular macromolecules in response to stress or starvation. Autophagy is carried out in four main phases: phagophore nucleation, autophagosome elongation, docking... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ATG13 |
![Immunocytochemistry/Immunofluorescence: ATG13 Antibody (3E4C8) [NBP2-52419] - Immunofluorescence analysis of Hela cells using ATG13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ATG13-Antibody-3E4C8-Immunofluorescence-NBP2-52419-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATG13 Antibody (3E4C8) [NBP2-52419] - Immunofluorescence analysis of Hela cells using ATG13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Flow Cytometry: ATG13 Antibody (3E4C8) [NBP2-52419] - Analysis of Hela cells using ATG13 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ATG13-Antibody-3E4C8-Flow-Cytometry-NBP2-52419-img0005.jpg "Flow Cytometry: ATG13 Antibody (3E4C8) [NBP2-52419] - Analysis of Hela cells using ATG13 mouse mAb (green) and negative control (red).")
![Western Blot: ATG13 Antibody (3E4C8) [NBP2-52419] - Western blot analysis using ATG13 mAb against human ATG13 (AA: 339-550) recombinant protein. (Expected MW is 48.9 kDa)](http://images.novusbio.com/fullsize/ATG13-Antibody-3E4C8-Western-Blot-NBP2-52419-img0002.jpg "Western Blot: ATG13 Antibody (3E4C8) [NBP2-52419] - Western blot analysis using ATG13 mAb against human ATG13 (AA: 339-550) recombinant protein. (Expected MW is 48.9 kDa)")
![ELISA: ATG13 Antibody (3E4C8) [NBP2-52419] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/ATG13-Antibody-3E4C8-ELISA-NBP2-52419-img0006.jpg "ELISA: ATG13 Antibody (3E4C8) [NBP2-52419] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);")
![Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Western Blot TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Data TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")