Novus Biologicals products are now on bio-techne.com

Arylsulfatase B/ARSB Antibody (708401)

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more
Arylsulfatase B/ARSB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
708401
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Arylsulfatase B/ARSB Antibody (708401) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB
Ser37-Met533
Accession # P15848
Specificity
Detects human Arylsulfatase B/ARSB in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ARSA, rhARSJ, or recombinant mouse ARSG is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ARSB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB4415 in the following applications:

Publications
Read Publications using
MAB4415 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arylsulfatase B/ARSB Antibody (708401)

  • ARSB
  • Arylsulfatase B
  • ASB
  • EC 3.1.6.12
  • G4S
  • MPS6
  • N-acetylgalactosamine-4-sulfatase

Background

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).

  1. Peters, C. et al. (1990) J. Biol. Chem. 265:3374.
  2. O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.
  3. Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-00154
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP1-90095
Species: Hu
Applications: IHC, IHC-P
H00000439-M03
Species: Hu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
NBP3-12295
Species: Hu, Pm, Rt
Applications: ELISA, IHC, IHC-P, IP, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP1-86616
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-87964
Species: Hu
Applications: IHC, IHC-P
NBP1-86687
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-82648
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-90095
Species: Hu
Applications: IHC, IHC-P
AF5787
Species: Hu, Mu
Applications: ICC, IHC, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-32899
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87511
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-91642
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-45731
Species: Ca, Hu, Pm
Applications: IHC, IHC-P, WB
H00347527-P01
Species: Hu
Applications: ELISA, AP, PA, WB
MAB4415
Species: Hu
Applications: WB, ICC/IF

Publications for Arylsulfatase B/ARSB Antibody (MAB4415)(4)

Review for Arylsulfatase B/ARSB Antibody (MAB4415) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4415:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Human 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22902879
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Arylsulfatase B/ARSB Antibody (MAB4415) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Verified Customer
02/20/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ARSB
Uniprot